Gazzeri S, Brambilla E, Jacrot M, Chauvin C, Benabid A L, Brambilla C
Groupe de Recherche sur le Cancer Bronchique, Pavillon, Université Joseph Fourier, Centre Hospitalier Albert Michallon, Grenoble, France.
Cancer Res. 1991 May 15;51(10):2566-71.
myc gene family activation (c-myc, L-myc, and N-myc) was examined in 26 human lung carcinomas and in their corresponding xenografts in nude mice. Of the 16 neuroendocrine (NE) carcinomas studied, amplification was observed in 4 with a c-myc probe and in 1 with both L- and N-myc probes. Overexpression was found in 1 of 7 cases studied for c-myc mRNA, in 1 of 7 cases for N-myc, and in 2 of 7 cases for L-myc. Of the 10 non-small cell lung carcinomas studied, only c-myc was amplified in 1 case and overexpressed in 5 of 7 cases. These results suggest that L- and N-myc gene activation are restricted to NE carcinomas. Over-expression of the myc gene without amplification was detected in 36% of cases. During heterotransplantation, there was a 27% change in myc gene abnormality and a 57% increase in myc expression levels, mostly in NE carcinomas (5 of 7; 71%). In a total of 42 xenografted lung carcinomas studied, 45% amplification and 77% overexpression of one of the myc genes were detected with a high prevalence of L-myc overexpression in NE carcinomas (50%) and of c-myc overexpression in non-small cell lung carcinomas (66%). Finally, 19 of 26 (73%) tumors are growing in nude mice with no myc gene amplification and 43% with no myc mRNA overexpression. Thus myc gene activation is not strictly required for heterotransplantation but seems to be a favorable factor in the maintenance and progression of lung carcinomas in vivo.
在26例人类肺癌及其相应的裸鼠异种移植瘤中检测了myc基因家族激活情况（c-myc、L-myc和N-myc）。在所研究的16例神经内分泌（NE）癌中，用c-myc探针检测到4例有扩增，用L-myc和N-myc探针检测到1例有扩增。在研究的7例c-myc mRNA病例中，有1例发现过表达；在7例N-myc病例中，有1例过表达；在7例L-myc病例中，有2例过表达。在所研究的10例非小细胞肺癌中，仅1例c-myc有扩增，7例中有5例过表达。这些结果表明，L-myc和N-myc基因激活仅限于NE癌。在36%的病例中检测到无扩增的myc基因过表达。在异种移植过程中，myc基因异常有27%的变化，myc表达水平增加57%，主要发生在NE癌中（7例中的5例；71%）。在总共42例异种移植肺癌中，检测到45%的myc基因之一有扩增，77%有过表达，其中NE癌中L-myc过表达发生率高（50%），非小细胞肺癌中c-myc过表达发生率高（66%）。最后，26例肿瘤中有19例（73%）在裸鼠中生长，无myc基因扩增，43%无myc mRNA过表达。因此，myc基因激活并非异种移植所严格必需，但似乎是体内肺癌维持和进展的一个有利因素。
