Curran F T, Allan R N, Keighley M R
General Hospital, Birmingham.
Gut. 1991 Apr;32(4):399-402. doi: 10.1136/gut.32.4.399.
Neutrophil superoxide anion production was measured in healthy subjects and in patients with quiescent and active Crohn's disease using superoxide dismutase inhibitable cytochrome C reduction. Three stimuli were used: phorbol 12-myristate 13-acetate (PMA1), phorbol 20-oxo-20-deoxy 12-myristate 13-acetate (PMA2), and Candida albicans in serum. Normal neutrophils produced significantly more superoxide anion than Crohn's disease neutrophils with both PMA1 (mean (SD) 9.6 (2.2) v 8.6 (1.8) nmol/10(6) cells/5 minutes, p = 0.04) and PMA2 (1.8 (0.8) v 0.8 (0.77) nmol/10(6) cells/5 minutes, p = 0.00004). With C albicans in serum, normal and Crohn's disease neutrophils produced similar amounts of superoxide anion (4.4 (1.5) v 4.3 (1.7) nmol/10(6) cells/30 minutes, not significant). Results were independent of disease activity. Superoxide anion production by PMA-stimulated Crohn's disease neutrophils is significantly lower than by normal neutrophils.
采用超氧化物歧化酶可抑制的细胞色素C还原法,测定了健康受试者以及静止期和活动期克罗恩病患者的中性粒细胞超氧阴离子生成情况。使用了三种刺激物:佛波醇12-肉豆蔻酸酯13-乙酸酯(PMA1)、佛波醇20-氧代-20-脱氧12-肉豆蔻酸酯13-乙酸酯(PMA2)以及血清中的白色念珠菌。无论是PMA1(平均值(标准差)9.6(2.2)对8.6(1.8)nmol/10⁶细胞/5分钟,p = 0.04)还是PMA2(1.8(0.8)对0.8(0.77)nmol/10⁶细胞/5分钟,p = 0.00004)刺激下,正常中性粒细胞产生的超氧阴离子均显著多于克罗恩病中性粒细胞。在血清中白色念珠菌刺激下,正常和克罗恩病中性粒细胞产生的超氧阴离子量相似(4.4(1.5)对4.3(1.7)nmol/10⁶细胞/30分钟,无显著差异)。结果与疾病活动度无关。PMA刺激的克罗恩病中性粒细胞产生超氧阴离子的能力显著低于正常中性粒细胞。