Nowlin D M, Cooper N R, Compton T
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
J Virol. 1991 Jun;65(6):3114-21. doi: 10.1128/JVI.65.6.3114-3121.1991.
Previous studies have demonstrated that human cytomegalovirus (HCMV) specifically binds to a fibroblast membrane glycoprotein(s) with a molecular mass from 30 to 34 kDa. In this study, the distribution of the putative receptor proteins was analyzed in a variety of cell types, including cell types representative of those that are infected in vivo. Using a sensitive microbinding assay (to score virus attachment) and an indirect detection method (to score HCMV-binding proteins), we found that the 34- and 32-kDa HCMV binding proteins are ubiquitous molecules, broadly distributed among diverse cell types. In addition, the level of virus attachment was found to correlate with the abundance of the 34- and 32-kDa cellular proteins, while the ability of the virus to penetrate cells and initiate infection did not. The results support the hypothesis that the 34- and 32-kDa cellular proteins represent the HCMV (attachment) receptor. The data also support the notion that additional cellular components are required for virus entry and fusion.
先前的研究表明，人类巨细胞病毒（HCMV）特异性结合分子量为30至34 kDa的成纤维细胞膜糖蛋白。在本研究中，分析了假定受体蛋白在多种细胞类型中的分布，包括代表体内受感染细胞类型的细胞。使用灵敏的微结合试验（用于评估病毒附着）和间接检测方法（用于评估HCMV结合蛋白），我们发现34 kDa和32 kDa的HCMV结合蛋白是普遍存在的分子，广泛分布于多种细胞类型中。此外，发现病毒附着水平与34 kDa和32 kDa细胞蛋白的丰度相关，而病毒穿透细胞并引发感染的能力则与之无关。这些结果支持以下假设：34 kDa和32 kDa细胞蛋白代表HCMV（附着）受体。数据还支持这样一种观点，即病毒进入和融合还需要其他细胞成分。
