Orimo S, Hiyamuta E, Arahata K, Sugita H
Department of Neurology, Kanto Teishin Hospital, Tokyo, Japan.
Muscle Nerve. 1991 Jun;14(6):515-20. doi: 10.1002/mus.880140605.
Immunohistochemical analysis of the inflammatory cells and complement C3 in the rat skeletal muscle was performed chronologically in bupivacaine-induced myonecrosis. At 30 minutes after injection, polymorphonuclear leukocytes appeared and increased in number, with a peak value at 12 hours, while macrophages reached the highest level at 2 days. In contrast, T cells comprised only a small population. Two weeks after the injection, all types of the inflammatory cells returned to the normal level. Deposition of complement C3 was recognized at 60 minutes at the surface membrane of degenerating muscle fiber. Our observation suggests the importance of both polymorphonuclear leukocytes and complement C3 in the early stage, and macrophages in the later stage of bupivacaine-induced myonecrosis. In addition, our findings cast doubt on the pathological significance of T cells in this model.
在布比卡因诱导的大鼠骨骼肌坏死中，按时间顺序对炎性细胞和补体C3进行了免疫组织化学分析。注射后30分钟，多形核白细胞出现且数量增加，在12小时达到峰值，而巨噬细胞在2天时达到最高水平。相比之下，T细胞仅占一小部分。注射后两周，所有类型的炎性细胞均恢复到正常水平。在变性肌纤维表面膜60分钟时可识别出补体C3的沉积。我们的观察结果表明，在布比卡因诱导的肌坏死早期，多形核白细胞和补体C3都很重要，而在后期巨噬细胞起重要作用。此外，我们的研究结果对该模型中T细胞的病理意义提出了质疑。
