Angeli José Pedro Friedmann, Ribeiro Lúcia Regina, Bellini Marilanda Ferreira, Mantovani Mário Sérgio
Laboratório de Genética Toxicológica, Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, Paraná 86951-990, Brazil.
Arch Toxicol. 2009 Jan;83(1):81-6. doi: 10.1007/s00204-008-0319-5. Epub 2008 Jun 5.
The mushroom Agaricus blazei is studied for its nutraceutical potential and as a medicinal supplement. The aim of the present study was to investigate the chemoprotective effect of beta-glucan extracted from the mushroom A. blazei against DNA damage induced by benzo[a]pyrene (B[a]P), using the comet assay (genotoxicity) and micronucleus assay with cytokinesis block (mutagenicity) in a human hepatoma cell line (HepG2). To elucidate the possible beta-glucan mechanism of action, desmutagenesis or bioantimutagenesis types, three treatment protocols were tested: simultaneous, pre-treatment, and presimultaneous. The results showed that beta-glucan does not exert genotoxic or mutagenic effect, but that it does protect against DNA damage caused by B[a]P in every protocol tested. The data suggest that beta-glucan acts through binding to B[a]P or the capture of free radicals produced during its activation. On the other hand, the pre-treatment results also suggest the possibility that beta-glucan modulates cell metabolism.
对姬松茸这种蘑菇的营养保健潜力及其作为药用补充剂进行了研究。本研究的目的是，在人肝癌细胞系（HepG2）中，使用彗星试验（遗传毒性）和胞质分裂阻滞微核试验（致突变性），研究从姬松茸中提取的β-葡聚糖对苯并[a]芘（B[a]P）诱导的DNA损伤的化学保护作用。为阐明β-葡聚糖可能的作用机制、去诱变或生物抗诱变类型，测试了三种处理方案：同时处理、预处理和预同时处理。结果表明，β-葡聚糖不会产生遗传毒性或诱变作用，但在每个测试方案中，它确实能保护细胞免受B[a]P引起的DNA损伤。数据表明，β-葡聚糖通过与B[a]P结合或捕获其激活过程中产生的自由基发挥作用。另一方面，预处理结果还表明β-葡聚糖可能调节细胞代谢。
