Fu Guang-Hua, Cheng Long-Fei, Shi Shao-Hua, Peng Chun-Xiang, Chen Hong-Mei, Huang Yu
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.
Bing Du Xue Bao. 2008 Jun;24(2):138-43.
To reveal the molecular biological characteristics of genome of circovirus in infected ducks, two nucleotide fragments were amplified by overlapping PCRs using DNA extracted from various tissues of ducks. After they had been assembled together, the nucleotide components, the genome organization and the phylogenetic scale of the sequence were analyzed. The results showed that the obtained sequence is a circular DNA with a total length of 1995nt. It contains 6 open reading frames (ORFs), and shares a high identity of 97.4% with the MuDCV circovirus sequence presented in GenBank (AY228555). These results indicate that the amplified product stems from duck circovirus sequence.
为揭示鸭圆环病毒感染鸭基因组的分子生物学特性，以鸭各组织提取的DNA为模板，通过重叠PCR扩增出两个核苷酸片段。将它们拼接在一起后，对该序列的核苷酸组成、基因组结构及系统发育关系进行了分析。结果显示，获得的序列为环状DNA，全长1995nt，含有6个开放阅读框（ORF），与GenBank中登录的MuDCV圆环病毒序列（AY228555）具有97.4%的高度同源性。这些结果表明，扩增产物源自鸭圆环病毒序列。
