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[反相高效液相色谱法同时测定青海野生川西獐牙菜中的四种成分]

[Simultaneous determination of four constituents in wild Gentiana lawrencei from Qinghai province by RP-HPLC].

作者信息

Zhang Xiaolong, Zhang Haitao, Ji Lanju

机构信息

Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Nov;34(22):2884-6.

PMID:20209951

Abstract

OBJECTIVE

To establish a RP-HPLC method for simultaneously determination of swertiamarin, gentiopicroside, sweroside, isoorientin in Gentiana lawrencei from Qinghai province.

METHOD

The RP-HPLC method was used. Chromatographic column was the Kromasil C18 column (4.6 mm x 250 mm, 5 microm). The gradient elution solvent system was composed of acetonitrile (A) and (0.1% H3PO4) water (B). The ratio of acetonitrile was as follows: 0.00-27.00 min, 10% -17% (A); 27.00-45.00 min, 17% -33% (A); 45.01-55.00 min, 100%-100% (A). The detective wavelength was 240 nm; the flow rate was 1 mL x min(-1); column temperature was set at 25 degrees C.

RESULT

Swertiamarin, gentiopicroside, sweroside and isoorientin were base-isolated. The method had good linearity within the ranges of 2.12-10.6 mg x mL(-1) for swertiamarin (r = 0.999 4), 2.46-12.3 g x L(-1) for gentiopicroside (r = 0.999 7), 2.47-12.4 g x L(-1) for sweroside (r = 0.999 2) and 0.309-1.54 mg x mL(-1) for isoorientin (r = 0.999 5).

CONCLUSION

The method is rapid, precise and repeatable, can be applied to control the quality of G. lawrencei.

摘要

目的

建立一种同时测定青海产川西獐牙菜中獐牙菜苦苷、龙胆苦苷、獐牙菜苷、异荭草苷含量的反相高效液相色谱法。

方法

采用反相高效液相色谱法。色谱柱为Kromasil C18柱（4.6 mm×250 mm，5μm）。梯度洗脱溶剂系统由乙腈（A）和（0.1%磷酸）水（B）组成。乙腈比例如下：0.00 - 27.00分钟，10% - 17%（A）；27.00 - 45.00分钟，17% - 33%（A）；45.01 - 55.00分钟，100% - 100%（A）。检测波长为240 nm；流速为1 mL·min⁻¹；柱温设定为25℃。

结果

獐牙菜苦苷、龙胆苦苷、獐牙菜苷和异荭草苷得到基线分离。该方法在獐牙菜苦苷2.12 - 10.6 mg·mL⁻¹范围内线性良好（r = 0.999 4），龙胆苦苷2.46 - 12.3 g·L⁻¹范围内线性良好（r = 0.999 7），獐牙菜苷2.47 - 12.4 g·L⁻¹范围内线性良好（r = 0.999 2），异荭草苷0.309 - 1.54 mg·mL⁻¹范围内线性良好（r = 0.999 5）。