[车前草中桃叶珊瑚苷的定性和定量分析]

[Qualitative and quantitative analysis of plantamajoside in Plantaginis Herba].

作者信息

Sun Qian, Geng Fang, Cheng Xuemei, Yang Li, Wang Zhengtao

机构信息

Department of Pharmacognosy, China Pharmaceutical University, Nanjing 211198, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2095-8.

PMID:21046737

Abstract

Plantamajoside is one of the main bioactive compounds in Plantaginis Herba A TLC method was developed identification of plantamajoside in 11 Plantaginis Herba samples using silica gel G as coating substance and a mixture of ethyl acetiate methanol-formic acid-water (18: 3 : 1.5 : 1) as a developing solvent, the established TLC condition displayed a very well separation on the chromatogram of tested Plantaginis Herba samples and the marker compound plantamajoside showed as a distinct light-blue fluorescence spot observed under UV 365 nm. Using the HPLC method, plantamajoside was separated at 30 degrees C on a Promocil C18, (4.6 mm x 250 mm, 5 microm) column with acetonitrile-0.1% formic acid (17:83) as the mobile phase. The detection wavelength was set at 330 nm and the flow rate was 1 mL x min(-1). The calibration curve of plantamajoside displayed ideal linearity over the range of 0.0499-11.9664 microg (r = 0.9999), and the average recovery of plantamajoside was 100.6% with a RSD of 2.7%. The contents of plantamajoside were in the range of 0.067%-1.80% in Plantaginis Herba The established TLC identification and HPLC were sensitive, reliable and repeatable, which can be applied for the quality evaluation and standard criteria of Plantaginis Herba.

摘要

大车前苷是车前草中的主要生物活性成分之一。建立了一种薄层色谱（TLC）方法，以硅胶G为涂布剂，乙酸乙酯 - 甲醇 - 甲酸 - 水（18∶3∶1.5∶1）的混合液为展开剂，用于11个车前草样品中大车前苷的鉴别。所建立的TLC条件在供试车前草样品的色谱图上显示出良好的分离效果，对照品大车前苷在紫外光365nm下呈现出明显的浅蓝色荧光斑点。采用高效液相色谱（HPLC）法，在Promocil C18（4.6mm×250mm，5μm）色谱柱上，于30℃，以乙腈 - 0.1%甲酸（17∶83）为流动相分离大车前苷。检测波长设定为330nm，流速为1mL·min⁻¹。大车前苷的校准曲线在0.0499 - 11.9664μg范围内呈现出理想的线性关系（r = 0.9999），大车前苷的平均回收率为100.6%，相对标准偏差（RSD）为2.7%。车前草中大车前苷的含量在0.067% - 1.80%之间。所建立的TLC鉴别和HPLC方法灵敏、可靠且具有重复性，可用于车前草的质量评价和标准制定。