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适配体作为治疗诊断试剂：修饰、血清稳定性和功能化。

Aptamers as theranostic agents: modifications, serum stability and functionalisation.

机构信息

School of Medicine, Deakin University, Pigdons Road, Waurn Ponds, Victoria 3217, Australia.

出版信息

Sensors (Basel). 2013 Oct 10;13(10):13624-37. doi: 10.3390/s131013624.

DOI:10.3390/s131013624
PMID:24152925
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3859083/
Abstract

Aptamers, and the selection process known as Systematic Evolution of Ligands by Exponential Enrichment (SELEX) used to generate them, were first described more than twenty years ago. Since then, there have been numerous modifications to the selection procedures. This review discusses the use of modified bases as a means of enhancing serum stability and producing effective therapeutic tools, as well as functionalising these nucleic acids to be used as potential diagnostic agents.

摘要

适体及其筛选技术系统进化的配体（SELEX）于二十多年前首次被描述。自那时以来，筛选程序已经进行了多次修改。本文讨论了使用修饰碱基来提高血清稳定性和产生有效治疗工具的方法，以及将这些核酸进行功能化以用作潜在诊断剂的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/12c23f879ef0/sensors-13-13624f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/aa6c90cedfbd/sensors-13-13624f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/fefd3c0aded8/sensors-13-13624f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/12c23f879ef0/sensors-13-13624f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/aa6c90cedfbd/sensors-13-13624f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/fefd3c0aded8/sensors-13-13624f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b5f/3859083/12c23f879ef0/sensors-13-13624f3.jpg

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2
Efficacy of base-modification on target binding of small molecule DNA aptamers.碱基修饰对小分子 DNA 适体与靶标结合的效力的影响。
J Am Chem Soc. 2013 Jun 26;135(25):9412-9. doi: 10.1021/ja4012222. Epub 2013 Jun 18.
3
Capillary electrophoresis-systematic evolution of ligands by exponential enrichment selection of base- and sugar-modified DNA aptamers: target binding dominated by 2'-O,4'-C-methylene-bridged/locked nucleic acid primer.
基于脱氧核酶的比色生物传感器用于快速检测福氏志贺菌。
Sci Rep. 2025 Apr 29;15(1):14942. doi: 10.1038/s41598-025-94494-0.
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The 20th Anniversary of Pegaptanib (MacugenTM), the First Approved Aptamer Medicine: History, Recent Advances and Future Prospects of Aptamers in Therapy.首个获批的适体药物——培加他尼（MacugenTM）二十周年：适体在治疗领域的历史、近期进展及未来前景
Pharmaceutics. 2025 Mar 20;17(3):394. doi: 10.3390/pharmaceutics17030394.
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Novel Aptamer Strategies in Combating Bacterial Infections: From Diagnostics to Therapeutics.对抗细菌感染的新型适配体策略：从诊断到治疗
Pharmaceutics. 2024 Aug 29;16(9):1140. doi: 10.3390/pharmaceutics16091140.
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Sci Rep. 2024 Jun 12;14(1):13552. doi: 10.1038/s41598-024-64559-7.
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Nat Rev Methods Primers. 2023;3. doi: 10.1038/s43586-023-00247-6. Epub 2023 Jul 20.
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