Burton L E, Ray R E, Bradford J R, Orr J P, Nickerson J A, Wells W W
J Nutr. 1976 Nov;106(11):1610-6. doi: 10.1093/jn/106.11.1610.
Neonatal rats of the Holtzman strain, 6 days of age, were fed a myo-inositol restricted liquid formula by gastric intubation for 10 days, after which they were fed a purified myo-inositol-free diet until they were 72 days old. No differences in weight gain were observed between myo-inositol/100 ml of formula or 150 mg myo-inositol/100 g diet. Most tissues examined from rats fed the myo-inositol deprived formula and diet had lower free myo-inositol levels than the controls with the exception of the liver. Despite reduced free and lipid-bound myo-inositol in the liver, there was no evidence of fatty liver in the young rats at any age. The cerebrum and cerebellum of myo-inositol deprived rats had normal myelination and mitochondriogenesis as judged by the levels of 2',3'-cyclic nucleotide-3'-phosphohydrolase (EC 3.1.4.1) and fumarase (EC 4.2.1.2) activity, respectively.
对6日龄的霍尔兹曼品系新生大鼠通过胃插管给予肌醇受限的液体配方食物，持续10天，之后给予纯化的无肌醇饮食直至72日龄。每100毫升配方食物含肌醇或每100克饮食含150毫克肌醇时，大鼠的体重增加没有差异。除肝脏外，与对照组相比，喂食肌醇缺乏的配方食物和饮食的大鼠所检测的大多数组织中游离肌醇水平较低。尽管肝脏中游离和脂质结合的肌醇减少，但在任何年龄的幼鼠中均未发现脂肪肝的证据。根据2',3'-环核苷酸-3'-磷酸水解酶（EC 3.1.4.1）和延胡索酸酶（EC 4.2.1.2）的活性水平判断，肌醇缺乏大鼠的大脑和小脑具有正常的髓鞘形成和线粒体生成。
