Burton L E, Wells W W
J Nutr. 1976 Nov;106(11):1617-28. doi: 10.1093/jn/106.11.1617.
Effects of dietary myo-inositol deprivation were examined during prenatal and postnatal development and during lactation in the rat. The deficient diet contained no detectable myo-inositol while the supplemented diet contained 0.5% (w/w) myo-inositol while the supplemented diet ct contained 0.5% (w/w) myo-inositol at the expense of sucrose. Both diets contained 25% casein, adequate amounts of all known vitamins, choline, and essential fatty acids as well as 0.5% (w/w) phthalylsulfathiazole to depress myo-inositol contribution to the diet by microorganisms. Pregnant rats of the Holtzman strain were fed the respective diets during gestation and lactation, and pups were fed the corresponding diet after weaning until 3 months of age. There were no significant differen-es in body weight between experimental groups. Supplementation of the diet with myo-inositol significanly increased the levels of myo-inositol in plasma, liver, kidney, and intestine of pups at all ages examined, and significantly increased the levels of myo-inositol in the milk and mammary tissue during lactation. During lactation, the myo-inositol deprived dams developed severe fatty livers (31% w/w) characterized by diminished phosphatidyl-inositol (50%) and total phospholipid phosphorus (57%) levels as compared with controls. After weaning, the liver lipid content of the myo-inositol deprived dams returned to normal (4.5%). The data suggest that a possible threshold level of free myo-inositol (approximately 0.15 mumoles/g lipid-free tissue) was required to prevent fatty liver in lactating dams under these dietary conditions. Effects of the deficient diet on fertility were also examined. Based on sperm count and production of offspring, there were no differnences between the experimental and control males. Females of both groups showed equal ability to produce offspring.
在大鼠的产前、产后发育及哺乳期，研究了膳食中肌醇缺乏的影响。缺乏肌醇的饮食中未检测到肌醇，而补充肌醇的饮食含有0.5%（w/w）的肌醇，补充肌醇的饮食中用蔗糖替代了等量的其他成分。两种饮食均含有25%的酪蛋白、所有已知维生素、胆碱和必需脂肪酸的适量含量，以及0.5%（w/w）的邻苯二甲酰磺胺噻唑，以抑制微生物对饮食中肌醇的贡献。将Holtzman品系的怀孕大鼠在妊娠和哺乳期喂食相应的饮食，幼崽在断奶后喂食相应的饮食直至3个月大。实验组之间的体重没有显著差异。在所有检测的年龄段，用肌醇补充饮食显著提高了幼崽血浆、肝脏、肾脏和肠道中的肌醇水平，并显著提高了哺乳期乳汁和乳腺组织中的肌醇水平。在哺乳期，缺乏肌醇的母鼠出现严重脂肪肝（31% w/w），其特征是与对照组相比，磷脂酰肌醇（50%）和总磷脂磷（57%）水平降低。断奶后，缺乏肌醇的母鼠肝脏脂质含量恢复正常（4.5%）。数据表明，在这些饮食条件下，可能需要一个游离肌醇的阈值水平（约0.15微摩尔/克无脂组织）来预防哺乳期母鼠的脂肪肝。还研究了缺乏肌醇的饮食对生育能力的影响。根据精子数量和后代产量，实验组和对照组雄性之间没有差异。两组雌性产生后代的能力相同。
