Riobó P, Paz B, Franco J M, Vázquez J A, Murado M A, Cacho E
Grupo de Fitoplancton Tóxico, Instituto Investigacións Mariñas (CSIC). Eduardo Cabello 6, 36208 Vigo, Galicia, Spain.
Food Chem Toxicol. 2008 Aug;46(8):2639-47. doi: 10.1016/j.fct.2008.04.020. Epub 2008 Apr 25.
Nowadays, a variety of protocols are applied to quantitate palytoxin. However, there is not desirable agreement among them, the confidence intervals of the basic toxicological parameters are too wide and the formal descriptions lack the necessary generality to establish comparisons. Currently, the mouse bioassay is the most accepted one to categorize marine toxins and it must constitute the reference for other methods. In the present work, the mouse bioassay for palytoxin is deeply analyzed and carefully described showing the initial symptoms of injected mice which are presented here in the first time. These symptoms clearly differ from the more common marine toxins described up to now. Regarding to the toxicological aspects two considerations are taking into account: (i) the empiric models based in the dose-death time relationships cause serious ambiguities and (ii) the traditional moving average method contains in its regular use any inaccuracy elements. Herein is demonstrated that the logistic equation and the accumulative function of Weibull's distribution (with the modifications proposed) generate satisfactory toxicological descriptions in all the respects.
如今，有多种方案被用于定量刺尾鱼毒素。然而，它们之间并未达成理想的一致，基本毒理学参数的置信区间过宽，且形式描述缺乏进行比较所需的普遍性。目前，小鼠生物测定法是对海洋毒素进行分类时最被认可的方法，它必须成为其他方法的参考标准。在本研究中，对刺尾鱼毒素的小鼠生物测定法进行了深入分析并详细描述，展示了首次在此呈现的注射小鼠的初始症状。这些症状明显不同于迄今为止所描述的更常见的海洋毒素。关于毒理学方面，考虑了两个因素：（i）基于剂量 - 死亡时间关系的经验模型会导致严重的模糊性，以及（ii）传统的移动平均法在常规使用中包含不准确因素。本文证明，逻辑方程和威布尔分布的累积函数（经过所提出的修正）在所有方面都能产生令人满意的毒理学描述。
