Thiruvengadam Vasumathi, Chitharanjan Arun B, Kumar Kennedy, Singaram Venkatesan
Orthodontics, Panimalar Medical College Hospital & Research Institute, Chennai, IND.
Orthodontics, Retired, Chennai, IND.
Cureus. 2022 Mar 28;14(3):e23574. doi: 10.7759/cureus.23574. eCollection 2022 Mar.
Evaluation of adhesion of on recycled orthodontic brackets is significant, as is the main causative factor in enamel demineralization and many clinicians, in their practice, resort to reconditioning of brackets, as it is cost-effective. Thus, this study aimed to evaluate and compare the adhesion of on new brackets of three different companies (Group I, Group II, and Group III) and brackets recycled by three different recycling methods (RC I: flame heating followed by acid bath; RC II: flame heating followed by ultrasonic cleaning and electropolishing; RC III: flame heating followed by sandblasting and electropolishing). Materials and methods: A total of 10 brackets from each group were incubated with 10 colony-forming units (CFUs) of in trypticase soy broth overnight. The brackets were then washed with phosphate-buffered saline and treated with 0.25% trypsin for 20 minutes followed by vertexing the solution to remove the adhered bacteria and then the solution was plated on the blood agar and incubated overnight. The total viable count of bacteria was quantified.
Comparing all the three groups and recycling methods, Group II brackets showed significantly more adhesion, Group I brackets showed lesser adhesion, and Group III brackets showed intermediate adhesion. When comparing recycling methods, all the three methods of recycling with all the three groups showed more bacterial adhesion than the control brackets, which was statistically insignificant (P > 0.05).
Recycled brackets showed more bacterial adhesion and electropolishing resulted in reduced bacterial adhesion.
评估再利用正畸托槽上的细菌黏附情况具有重要意义，因为细菌是牙釉质脱矿的主要致病因素，并且许多临床医生在实践中会采用托槽再处理的方法，因为这样具有成本效益。因此，本研究旨在评估和比较三种不同公司的新托槽（第一组、第二组和第三组）以及通过三种不同回收方法回收的托槽（RC I：火焰加热后酸浴；RC II：火焰加热后超声清洗和电解抛光；RC III：火焰加热后喷砂和电解抛光）上的细菌黏附情况。材料与方法：每组共10个托槽在胰蛋白酶大豆肉汤中与10个菌落形成单位（CFU）的细菌一起孵育过夜。然后用磷酸盐缓冲盐水冲洗托槽，并用0.25%的胰蛋白酶处理20分钟，随后振荡溶液以去除黏附的细菌，然后将溶液接种在血琼脂上并孵育过夜。对细菌的总活菌数进行定量。
比较所有三组和回收方法，第二组托槽显示出明显更多的黏附，第一组托槽显示较少的黏附，第三组托槽显示中等程度的黏附。比较回收方法时，所有三组的三种回收方法均显示比对照托槽有更多的细菌黏附，但差异无统计学意义（P>0.05）。
回收的托槽显示出更多的细菌黏附，电解抛光可减少细菌黏附。
