Yazdanfar Siavash, Kenny Kevin B, Tasimi Krenar, Corwin Alex D, Dixon Elizabeth L, Filkins Robert J
GE Global Research, One Research Circle, Niskayuna, N.Y. 12309, USA.
Opt Express. 2008 Jun 9;16(12):8670-7. doi: 10.1364/oe.16.008670.
A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.
展示了一种用于数字显微镜的基于图像的简单自动聚焦方案，该方案使用少至两个中间图像就能使样品聚焦。该算法适用于商用倒置显微镜，并用于自动进行组织病理学组织切片的明场和荧光成像。
