Zhou Yu-Bo, Cao Jia-Bing, Wan Bing-Bing, Wang Xin-Rong, Ding Guo-Hui, Zhu Hong, Yang Hong-Meng, Wang Ke-Sheng, Zhang Xin, Han Ze-Guang
Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai, 201203, China.
Mol Cell Biochem. 2008 Oct;317(1-2):61-8. doi: 10.1007/s11010-008-9809-2. Epub 2008 Jun 12.
This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Furthermore, the secretion was not dependent on its predicted signal peptide. All the experiment results suggested that the secretion was a non-classical export. Phylogenetic analysis showed that the human BolAs belong to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among hBolA1, hBolA2, and hBolA3 could be responsible for their functional divergence. Our data provided a basis for functional studies of BolA protein family.
本研究报道，通过生物信息学和分子生物学实验鉴定出的所有三种人类BolA蛋白（hBolA1、hBolA2和hBolA3）都是新型非经典分泌蛋白。三种带有c-Myc标签的BolA融合蛋白可以分泌到转染的Cos-7细胞的培养基中，尽管它们不能与高尔基体共定位。而且，经BFA处理后，三种BolA蛋白的分泌并未受到抑制。此外，其分泌不依赖于预测的信号肽。所有实验结果表明，这种分泌是一种非经典输出。系统发育分析表明，人类BolA蛋白属于BolA亚家族功能有差异的三个不同组，其中hBolA1、hBolA2和hBolA3之间不同的螺旋-转角-螺旋基序可能是其功能差异的原因。我们的数据为BolA蛋白家族的功能研究提供了基础。
