Kaspar Katharina, Matziolis Georg, Strube Patrick, Sentürk Ufuk, Dormann Svenja, Bail Hermann J, Duda Georg N
Musculoskeletal Research Center Berlin, Center for Musculoskeletal Surgery, Charité, Universitätsmedizin Berlin, Free and Humboldt-University of Berlin, Augustenburger Platz 1, Forum 4, Psf. 24, D-13353 Berlin, Germany.
J Orthop Res. 2008 Dec;26(12):1649-55. doi: 10.1002/jor.20651.
A new small animal model of bone atrophic nonunion was established for investigating the process of bone regeneration by performing cauterization of the periosteum, removal of the local bone marrow, and stabilization with external fixation. The model allows the creation of an atrophic nonunion without the need for a critical size defect. Furthermore, it provides reproducible, well-defined mechanical conditions and minimized physical interference of the implant with the biological processes in the healing zone. Eighty adult Sprague-Dawley rats received an osteotomy of the left femur, stabilized with an external fixator. In half of the animals, the periosteum proximal and distal to the osteotomy was destroyed by cauterization and the adjacent bone marrow was removed (nonunion group). At 2 and 8 weeks after surgery, radiological, biomechanical, histological, and histomorphometrical analyses showed a typical physiological healing in the control group, while the nonunion group was characterized by resorption of the bone ends with some callus formation distant to the osteotomy. At both time points, the callus was composed of significantly less bone and significantly more connective tissue (p < 0.001). In addition, the torsional strength of the osteotomized femur was significantly less in the nonunion group than in the control group, which was comparable to that of the intact femur (p < 0.001). In conclusion, the present model allows the induction of an atrophic nonunion without the need of a critical size defect. It is reproducible, provides standardized biomechanical conditions, and allows minimized interaction of the implant with the healing zone.
通过对骨膜进行烧灼、去除局部骨髓并采用外固定进行稳定处理，建立了一种新的骨萎缩性骨不连小动物模型，用于研究骨再生过程。该模型无需制造临界尺寸骨缺损即可形成萎缩性骨不连。此外，它提供了可重复、明确的力学条件，并将植入物对愈合区域生物过程的物理干扰降至最低。80只成年Sprague-Dawley大鼠接受左股骨截骨术，并用外固定器固定。在一半的动物中，截骨术近端和远端的骨膜通过烧灼破坏，相邻骨髓被去除（骨不连组）。术后2周和8周，影像学、生物力学、组织学和组织形态计量学分析显示，对照组呈现典型的生理性愈合，而骨不连组的特征是骨端吸收，截骨处远处有一些骨痂形成。在两个时间点，骨痂中的骨组织明显较少，结缔组织明显较多（p<0.001）。此外，骨不连组截骨后股骨的扭转强度明显低于对照组，与完整股骨相当（p<0.001）。总之，本模型无需临界尺寸骨缺损即可诱导形成萎缩性骨不连。它具有可重复性，提供标准化的生物力学条件，并使植入物与愈合区域的相互作用最小化。
