Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang District, Beijing, 100020, China.
Department of Handsurgery, Tangshan Second Hospital, Tangshan, 063000, China.
J Orthop Surg Res. 2021 Apr 14;16(1):261. doi: 10.1186/s13018-021-02408-3.
Many non-union animal models have been developed to explore the problems surrounding fracture healing. However, the existing models are not perfect and cannot satisfy all non-union studies. This study aimed to make a non-union model of the tibia in rats by cauterization of the posterior of 2 mm on both sides of the fracture end after open osteotomy of the tibia and fixing the fractured tibia with a Kirschner wire 0.8 mm in diameter.
For this study, 96 female adult Sprague-Dawley (SD) rats were used. The rats underwent surgery to produce a tibial open fracture and were fixed with a 0.8-mm diameter Kirschner wire. In 48 of the rats, the periosteum proximal and distal to the fracture end was cauterized.
At 2, 4, 6, and 8 weeks after surgery, radiological and histological analysis showed typical physiological healing in the control group, and the healing rate was 100% at 6 weeks. But the non-union group was characterized by resorption of the fracture ends with few callus formations and no bridging callus formation, and the healing rate was 0% at 8 weeks.
This method represents a reproducible model to create atrophic non-unions. This model provides a new option for studying the basic healing mechanisms and evaluating new therapies for bone regeneration and treatment of non-unions.
为了探索骨折愈合相关问题，已经开发出许多非愈合动物模型。然而，现有的模型并不完善，无法满足所有非愈合研究的需要。本研究旨在通过对胫骨开放性骨折后骨折端两侧各 2mm 处进行烧灼，并使用直径 0.8mm 的克氏针固定骨折胫骨，从而建立大鼠胫骨非愈合模型。
本研究使用了 96 只成年雌性 Sprague-Dawley（SD）大鼠。大鼠接受手术以产生胫骨开放性骨折，并使用直径 0.8mm 的克氏针固定。在 48 只大鼠中，烧灼骨折端近端和远端的骨膜。
术后 2、4、6 和 8 周，影像学和组织学分析显示对照组出现典型的生理性愈合，6 周时愈合率为 100%。但是，非愈合组的特征是骨折端吸收，形成的骨痂较少，没有桥接骨痂形成，8 周时愈合率为 0%。
该方法代表了一种可复制的建立萎缩性非愈合的模型。该模型为研究基本愈合机制以及评估骨再生和治疗非愈合的新疗法提供了新的选择。
