Department of Bioelectronics and Biosensors, Alagappa University, Karaikudi 630003, India.
Department of Engineering, University of Messina, 98166 Messina, Italy.
Biosensors (Basel). 2020 Apr 2;10(4):33. doi: 10.3390/bios10040033.
The development of MnO-graphene (MnOGR) composite by microwave irradiation method and its application as an electrode material for the selective determination of serotonin (SE), popularly known as "happy chemical", is reported. Anchoring MnO nanoparticles on graphene, yielded MnO-GR composite with a large surface area, improved electron transport, high conductivity and numerous channels for rapid diffusion of electrolyte ions. The composite was characterized by X-ray photoelectron spectroscopy (XPS), Raman spectroscopy and scanning electron microscopy (SEM) for assessing the actual composition, structure and morphology. The MnO-GR composite modified glassy carbon electrode (GCE) exhibited an excellent electrochemical activity towards the detection of SE in phosphate buffer saline (PBS) at physiological pH of 7.0. Under optimum conditions, the modified electrode could be applied to the quantification of serotonin by square wave voltammetry over a wide linear range of 0.1 to 800 µM with the lowest detection limit of 10 nM (S/N = 3). The newly fabricated sensor also exhibited attractive features such as good anti-interference ability, high reproducibility and long-term stability.
报道了通过微波辐射法制备 MnO-石墨烯（MnOGR）复合材料，并将其作为电极材料应用于选择性测定 5-羟色胺（SE），俗称“快乐化学物质”。MnO 纳米粒子锚定在石墨烯上，得到具有大表面积、改善电子传递、高导电性和大量电解质离子快速扩散通道的 MnO-GR 复合材料。通过 X 射线光电子能谱（XPS）、拉曼光谱和扫描电子显微镜（SEM）对复合材料进行了表征，以评估实际组成、结构和形态。MnO-GR 复合材料修饰的玻碳电极（GCE）在生理 pH 值为 7.0 的磷酸盐缓冲盐（PBS）中对 SE 的检测表现出优异的电化学活性。在最佳条件下，修饰电极可通过方波伏安法对 0.1 至 800 μM 宽线性范围内的 5-羟色胺进行定量，检测限低至 10 nM（S/N = 3）。新制备的传感器还具有良好的抗干扰能力、高重现性和长期稳定性等吸引人的特点。
