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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Vogel-Mikuš Katarina, Regvar Marjana, Mesjasz-Przybyłowicz Jolanta, Przybyłowicz Wojciech J, Simčič Jure, Pelicon Primož, Budnar Miloš

Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia.

Materials Research Group, iThemba LABS, PO Box 722, Somerset West 7129, South Africa.

New Phytol. 2008;179(3):712-721. doi: 10.1111/j.1469-8137.2008.02519.x. Epub 2008 Jun 28.

Localization of cadmium (Cd) and other elements was studied in the leaves of the field-collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro-PIXE (proton-induced X-ray emission). * The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole-leaf cross-sections and selected tissues were obtained. * Cd was preferentially localized in the lower epidermis (820 microg g(-1) DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll. * Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.

使用微束质子激发X射线发射分析（micro-PIXE）技术，对采自斯洛文尼亚一处铅矿和冶炼厂附近重金属污染区域的野外镉/锌（Cd/Zn）超富集植物早花遏蓝菜（Thlaspi praecox）叶片中的镉（Cd）及其他元素进行了定位研究。* 样品采用冷冻固定法制备。获得了整叶横切面及选定组织的定量元素图谱和平均浓度。* Cd优先定位在叶片下表皮（820 μg g⁻¹ DW）、维管束和上表皮，而在叶肉中的浓度约为下表皮的两倍。* 考虑到叶肉与表皮相比体积较大，叶肉被认为是一个相对较大的Cd储存库，可能在组织和细胞水平参与Cd的解毒/稀释过程。

Vogel-Mikuš Katarina, Regvar Marjana, Mesjasz-Przybyłowicz Jolanta, Przybyłowicz Wojciech J, Simčič Jure, Pelicon Primož, Budnar Miloš

Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia.

Materials Research Group, iThemba LABS, PO Box 722, Somerset West 7129, South Africa.

New Phytol. 2008;179(3):712-721. doi: 10.1111/j.1469-8137.2008.02519.x. Epub 2008 Jun 28.

Localization of cadmium (Cd) and other elements was studied in the leaves of the field-collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro-PIXE (proton-induced X-ray emission). * The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole-leaf cross-sections and selected tissues were obtained. * Cd was preferentially localized in the lower epidermis (820 microg g(-1) DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll. * Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.

使用微束质子激发X射线发射分析（micro-PIXE）技术，对采自斯洛文尼亚一处铅矿和冶炼厂附近重金属污染区域的野外镉/锌（Cd/Zn）超富集植物早花遏蓝菜（Thlaspi praecox）叶片中的镉（Cd）及其他元素进行了定位研究。* 样品采用冷冻固定法制备。获得了整叶横切面及选定组织的定量元素图谱和平均浓度。* Cd优先定位在叶片下表皮（820 μg g⁻¹ DW）、维管束和上表皮，而在叶肉中的浓度约为下表皮的两倍。* 考虑到叶肉与表皮相比体积较大，叶肉被认为是一个相对较大的Cd储存库，可能在组织和细胞水平参与Cd的解毒/稀释过程。