Yaltirik Mehmet, Ozbas Hakan, Bilgic Bilge, Issever Halim
Faculty of Dentistry, Department of Oral Surgery and Medicine, Istanbul University, Turkey.
J Endod. 2004 Feb;30(2):95-9. doi: 10.1097/00004770-200402000-00008.
The purpose of this study was to evaluate the subcutaneous connective tissue reactions to ProRoot, mineral trioxide aggregate (MTA; Dentsply), and Oralloy, high-copper amalgam (Coltene). These materials were placed in polyethylene tubes and implanted into dorsal connective tissue of Wistar albino rats, and tissue biopsies were collected and histologically examined 7, 15, 30, 60, and 90 days after the implantation procedure. The presence of inflammation, predominant cell type, calcification, and thickness of fibrous connective tissue were recorded. Scores were defined as follows: 0, none or few inflammatory cells, no reaction; 1, < 25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, > or = 125 cells, severe reaction. Fibrous capsule was categorized as "thin" when thickness was < 150 microm and "thick" at > 150 microm. Necrosis and formation of calcification were recorded. Both materials were well tolerated by the tissues in a 90-day evaluation period. One notable finding is the presence of dystrophic calcification in connective tissue adjacent to MTA; this finding is consistent with the hypothesis of hard tissue induction by this material.
本研究的目的是评估皮下结缔组织对ProRoot、三氧化矿物凝聚体（MTA；登士柏公司）和高铜银汞合金（Oralloy；科尔tene公司）的反应。将这些材料置于聚乙烯管中，植入Wistar白化大鼠的背部结缔组织，在植入手术后7、15、30、60和90天收集组织活检样本并进行组织学检查。记录炎症的存在、主要细胞类型、钙化情况以及纤维结缔组织的厚度。评分定义如下：0分，无或仅有少量炎性细胞，无反应；1分，<25个细胞，轻度反应；2分，25至125个细胞，中度反应；3分，≥125个细胞，重度反应。当纤维囊厚度<150微米时归类为“薄”，>150微米时归类为“厚”。记录坏死和钙化的形成情况。在90天的评估期内，两种材料均被组织良好耐受。一个值得注意的发现是，在与MTA相邻的结缔组织中存在营养不良性钙化；这一发现与该材料诱导硬组织形成的假说一致。
