Lu Wei-Hong, Wang Xin-Zhu, Zheng Qi, Guan Shuang-Hong, Xin Ping, Sun Ye-Qing
Department of Astronautic Engineering, Harbin Institute of Technology, Harbin 150001, China.
Genomics Proteomics Bioinformatics. 2008 Mar;6(1):51-60. doi: 10.1016/S1672-0229(08)60020-0.
To further study the characteristics of changes on the molecular level of rice mutants induced in space environment, we analyzed proteins in leaves and seeds of four rice mutants (two high-tillering and two low-tillering) in the 8(th) and 9(th) generations after a 15-day spaceflight, and compared with their ground controls by two-dimentional polyacrylamide gel electrophoresis and reverse phase liquid chromatography (RPLC). In addition, the albumin, globulin, prolamine, glutelin, and amylose of the mutant seeds were analyzed by RPLC and ultra-violet spectrometry. The results showed that the low-abundance proteins of leaves in the peak tillering stage are more likely to be induced compared with their corresponding controls. The albumin, globulin, and prolamine of the mutant seeds revealed changes when compared with their controls, and the characteristics of changes in different mutants were stably inherited in the 8(th) and 9(th) generations, suggesting that they can be used as bio markers to identity the mutants induced by spaceflight. Moreover, two proteins (SSP9111 and SSP6302) were found to be expressed with high intensity (two-fold change) in different mutants, which were both correlated with photosystem according to mass spectrometry and database searching.
为进一步研究空间环境诱导的水稻突变体分子水平变化特征，我们对4个水稻突变体（2个高分蘖和2个低分蘖）经15天太空飞行后第8代和第9代的叶片和种子中的蛋白质进行了分析，并通过二维聚丙烯酰胺凝胶电泳和反相液相色谱（RPLC）与地面对照进行比较。此外，通过RPLC和紫外光谱对突变体种子中的清蛋白、球蛋白、醇溶蛋白、谷蛋白和直链淀粉进行了分析。结果表明，与相应对照相比，分蘖盛期叶片中的低丰度蛋白质更容易被诱导。突变体种子的清蛋白、球蛋白和醇溶蛋白与对照相比有变化，且不同突变体的变化特征在第8代和第9代中稳定遗传，表明它们可作为鉴定太空飞行诱导突变体的生物标志物。此外，发现两种蛋白质（SSP9111和SSP6302）在不同突变体中高强度表达（两倍变化），根据质谱和数据库搜索，它们均与光系统相关。
