呼肠孤病毒mRNA中封闭和未封闭5'-末端的差异合成：焦磷酸和焦磷酸酶的作用
Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.
作者信息
Furuichi Y, Shatkin A J
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3448-52. doi: 10.1073/pnas.73.10.3448.
Abstract
Viral mRNA synthesized in vitro by reovirusassociated enzymes contained 5'-terminal ppG, GpppG, or m7GpppGm. Selective synthesis of one type of 5'-end was obtained by adjusting the composition of the transcription reaction mixture based on a series of reactions proposed for formation of blocked, methylated 5'-terminal sequences of mRNA (capformation). The presence of inorganic pyrophosphate increased the proportion of ppG 5'-ends, while addition of pyrophosphatase yielded mRNA with 5'-terminal GpppG, which in the presence of a methyl donor was converted to m7GpppGm.
摘要
呼肠孤病毒相关酶在体外合成的病毒信使核糖核酸（mRNA）含有5'-末端的三磷酸鸟苷（ppG）、鸟苷四磷酸（GpppG）或7-甲基鸟苷四磷酸（m7GpppGm）。基于一系列提出的用于形成封闭的、甲基化的mRNA 5'-末端序列（帽形成）的反应，通过调整转录反应混合物的组成，可选择性合成一种类型的5'-末端。无机焦磷酸的存在增加了ppG 5'-末端的比例，而添加焦磷酸酶则产生具有5'-末端GpppG的mRNA，在甲基供体存在的情况下，其可转化为m7GpppGm。
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Differential synthesis of blocked and unblocked 5'-termini in reovirus mRNA: effect of pyrophosphate and pyrophosphatase.呼肠孤病毒mRNA中封闭和未封闭5'-末端的差异合成：焦磷酸和焦磷酸酶的作用
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1
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Nucleic Acids Res. 1974 Jun;1(6):809-22. doi: 10.1093/nar/1.6.809.
2
Transcription by infectious subviral particles of reovirus.呼肠孤病毒感染性子病毒颗粒的转录
J Virol. 1972 Oct;10(4):698-706. doi: 10.1128/JVI.10.4.698-706.1972.
3
The methylation of adenovirus-specific nuclear and cytoplasmic RNA.腺病毒特异性核RNA和胞质RNA的甲基化
Nucleic Acids Res. 1976 Mar;3(3):749-65. doi: 10.1093/nar/3.3.749.
4
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Nature. 1976 Mar 25;260(5549):359-60. doi: 10.1038/260359a0.
5
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