一种将肽和蛋白质靶向附着于固体支持物的方法。
An approach to the targeted attachment of peptides and proteins to solid supports.
作者信息
Hofmann K, Kiso Y
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3516-8. doi: 10.1073/pnas.73.10.3516.
Abstract
A novel approach to affinity columns is described that is based on the high avidity of biotinylated molecules for avidin attached to solid supports. Biocytin amide [Nepsilon-(+)-biotinyllysine amide] was coupled to the COOH-terminal carboxyl group of corticotropin(1-24) [ACTH(1-24)] to form [biocytin25]ACTH(1-25) amide. The ability of this peptide to stimulate steroidogenesis of bovine adrenocortical cells was within experimental error identical to that of ACTH(1-24). The peptide also binds to avidin and avidin-Sepharose, forming stable complexes. Thus, with biotin as the anchor, the adrenocorticotropically active segment of the ACTH molecule was attached to a solid support in a targeted manner. The general applicability of this principle for the attachment of peptides and proteins to solid supports is discussed.
摘要
描述了一种基于生物素化分子对附着在固体支持物上的抗生物素蛋白具有高亲和力的新型亲和柱方法。生物胞素酰胺[Nε-(+)-生物素基赖氨酸酰胺]与促肾上腺皮质激素(1-24)[促肾上腺皮质激素(1-24)]的COOH末端羧基偶联，形成[生物胞素25]促肾上腺皮质激素(1-25)酰胺。该肽刺激牛肾上腺皮质细胞类固醇生成的能力在实验误差范围内与促肾上腺皮质激素(1-24)相同。该肽还与抗生物素蛋白和抗生物素蛋白-琼脂糖结合，形成稳定的复合物。因此，以生物素为锚，促肾上腺皮质激素分子的促肾上腺皮质活性片段以靶向方式附着在固体支持物上。讨论了该原理在将肽和蛋白质附着到固体支持物上的一般适用性。
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Proc Natl Acad Sci U S A. 1976 Oct;73(10):3516-8. doi: 10.1073/pnas.73.10.3516.
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本文引用的文献
1
Studies on polypeptides. XXXIV. Enzymic properties of partially synthetic De(16-20)- and De(15-20)-ribonucleases S'1-3.多肽研究。三十四。部分合成的缺失（16 - 20）和缺失（15 - 20）核糖核酸酶S'1 - 3的酶学性质
J Am Chem Soc. 1966 Aug 5;88(15):3633-9. doi: 10.1021/ja00967a030.
2
Sepharose-avidin column for the binding of biotin or biotin-containing peptides.用于结合生物素或含生物素肽的琼脂糖-抗生物素蛋白柱。
Experientia. 1970 Mar 15;26(3):327. doi: 10.1007/BF01900128.
3
Synthetic studies of structure-function relationships in staphylococcal nuclease. Synthetic analogues of fragment P2.葡萄球菌核酸酶结构-功能关系的合成研究。片段P2的合成类似物。
J Biol Chem. 1969 Dec 10;244(23):6316-22.
4
Isolated adrenal cortex cells: ACTH agonists, partial agonists, antagonists; cyclic AMP and corticosterone production.分离的肾上腺皮质细胞：促肾上腺皮质激素激动剂、部分激动剂、拮抗剂；环磷酸腺苷和皮质酮生成
Arch Biochem Biophys. 1973 Jan;154(1):230-9. doi: 10.1016/0003-9861(73)90053-2.
5
Correlation of adrenocorticotropic activity of ACTH analogs with degree of binding to an adrenal cortical particulate preparation.促肾上腺皮质激素（ACTH）类似物的促肾上腺皮质活性与和肾上腺皮质微粒体制剂结合程度的相关性。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):829-36. doi: 10.1073/pnas.67.2.829.
6
ACTH diazotized to agarose: effects on isolated adrenal cells.重氮化到琼脂糖的促肾上腺皮质激素：对分离肾上腺细胞的影响
Biochem Biophys Res Commun. 1971 May 21;43(4):793-9. doi: 10.1016/0006-291x(71)90686-3.
7
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Biochem Biophys Res Commun. 1968 Sep 6;32(5):806-10. doi: 10.1016/0006-291x(68)90312-4.
8
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9
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10
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