Rudolph D L, Yee J, Mone J, Foung S K, Lipka J J, Reyes G R, Hadlock K, Chan L, Villinger F, Lairmore M D
Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1992 Apr;30(4):858-61. doi: 10.1128/jcm.30.4.858-861.1992.
Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21env) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-I (rgp46env), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21env, none reacted with rgp46env, for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19gag), none of the specimens reacted with HTLV-II-specific epitope (gp52env). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type-specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.
从旧大陆猴的不同物种采集血清标本，根据人类嗜T淋巴细胞病毒（HTLV）感染情况分为血清阳性（n = 97）或血清阴性（n = 23），采用重组env加样的Western免疫印迹法和合成肽法进行检测，以同时检测和鉴别猴嗜T淋巴细胞病毒（STLV）感染。在97份血清阳性标本中，93份与重组跨膜（r21env）蛋白反应，90份与源自HTLV-I外膜糖蛋白中央区域的重组蛋白MTA-1（rgp46env）反应，因此检测灵敏度分别为96%和93%。在23份阴性猴标本中，1份与r21env反应，但无一与rgp46env反应，总体特异性分别为96%和100%。基于合成肽的免疫分析表明，97份血清阳性标本中有85份（88%）与HTLV-I特异性表位（p19gag）反应，但无一标本与HTLV-II特异性表位（gp52env）反应。这些结果表明，重组包膜加样的Western印迹法为STLV-I感染的血清学确认提供了一种简单方法，并且型特异性合成肽可用于确认血清阳性猴标本中的病毒类型。
