Seidel Albrecht, Spickenheuer Anne, Straif Kurt, Rihs Hans-Peter, Marczynski Boleslaw, Scherenberg Michael, Dettbarn Gerhard, Angerer Jürgen, Wilhelm Michael, Brüning Thomas, Jacob Jürgen, Pesch Beate
Biochemisches Institut für Umweltcarcinogene Prof Dr Gernot Grimmer-Stiftung, Grosshansdorf, Germany.
J Toxicol Environ Health A. 2008;71(11-12):734-45. doi: 10.1080/15287390801985265.
Polycyclic aromatic hydrocarbons (PAH) are metabolized in a complex manner. Although biological activity is associated with diol-epoxide formation, phenolic metabolites have predominantly been used in human biomonitoring. In this study monohydroxylated and new metabolites were characterized as biomarkers for occupational PAH exposure. In 97 male workers, personal exposure to 16 airborne PAH compounds was measured during shift. In postshift urine, 1-hydroxypyrene and 1,6- and 1,8-dihydroxypyrene (1-OHP, DiOHP) were determined as metabolites of pyrene (P), and the sum of 1-, 2-, 3-, 4-, and 9-hydroxyphenanthrenes (OHPHE), and PHE-dihydrodiols (PHED) as metabolites of phenanthrene (PHE). The referent group comprised 21 nonsmoking construction workers. Median (interquartile range) shift concentrations of airborne P and PHE were 1.46 (0.62-4.05 microg/m(3)) and 10.9 (3.69-23.77 microg/m(3)), respectively. The corresponding parameters were 3.86 (2.08-7.44) microg/g creatinine (crn) for 1-OHP, 0.66 (0.17-1.65) microg/g crn for DiOHP, 11.44 (5.21-34.76) microg/g crn for OHPHE, and 12.28 (3.3-97.76) microg/g crn for PHED in PAH-exposed workers. The median levels of 1-OHP and OHPHE were 0.09 (0.08-0.17 microg/m(3)) and 0.59 (0.45-1.39 microg/m(3)), respectively, in the referents. PHE correlated significantly with OHPHE and PHED, and P with 1-OHP but not with DiOHP. Under a doubling of PHE, OHPHE increased by a factor of 1.56 and PHED by 1.57. With a doubling of P, 1-OHP rose by 1.31 and DiOHP by 1.27. P is predominantly metabolized into 1-OHP, whereas PHE is metabolized equally into OHPHE and PHED. Thus metabolites of PHE were found as reliable biomarkers for PAH exposure.
多环芳烃（PAH）的代谢方式复杂。虽然生物活性与二醇环氧化物的形成有关，但酚类代谢产物主要用于人体生物监测。在本研究中，单羟基化和新的代谢产物被表征为职业性PAH暴露的生物标志物。对97名男性工人在轮班期间空气中16种PAH化合物的个人暴露情况进行了测量。在轮班后的尿液中，测定了芘（P）的代谢产物1-羟基芘以及1,6-和1,8-二羟基芘（1-OHP、DiOHP），以及菲（PHE）的代谢产物1-、2-、3-、4-和9-羟基菲（OHPHE）和菲二氢二醇（PHED）的总和。参照组包括21名不吸烟的建筑工人。空气中P和PHE的轮班浓度中位数（四分位间距）分别为1.46（0.62 - 4.05微克/立方米）和10.9（3.69 - 23.77微克/立方米）。PAH暴露工人中，1-OHP的相应参数为3.86（2.08 - 7.44）微克/克肌酐（crn），DiOHP为0.66（0.17 - 1.65）微克/克crn，OHPHE为11.44（5.21 - 34.76）微克/克crn，PHED为12.28（3.3 - 97.76）微克/克crn。参照组中1-OHP和OHPHE的中位数水平分别为0.09（0.08 - 0.17微克/立方米）和0.59（0.45 - 1.39微克/立方米）。PHE与OHPHE和PHED显著相关，P与1-OHP显著相关，但与DiOHP不相关。PHE浓度翻倍时，OHPHE增加1.56倍，PHED增加1.57倍。P浓度翻倍时，1-OHP上升1.31倍，DiOHP上升1.27倍。P主要代谢为1-OHP，而PHE则等量代谢为OHPHE和PHED。因此，PHE的代谢产物被发现是PAH暴露的可靠生物标志物。
