通过基质辅助激光解吸/电离四极杆离子阱飞行时间成像质谱法对转移膜上的糖鞘脂进行高灵敏度分析。
High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes.
作者信息
Goto-Inoue Naoko, Hayasaka Takahiro, Sugiura Yuki, Taki Takao, Li Yu-Teh, Matsumoto Mineo, Setou Mitsutoshi
机构信息
Department of Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Shizuoka 431-3192, Japan.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jul 1;870(1):74-83. doi: 10.1016/j.jchromb.2008.06.001. Epub 2008 Jun 6.
Abstract
Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).
摘要
糖鞘脂是细胞中普遍存在的成分。然而，糖鞘脂的分析技术仍有改进的空间。在此，我们报告了一种高灵敏度且便捷的分析技术，即利用成像质谱对糖鞘脂进行详细的结构分析。通过在聚偏二氟乙烯（PVDF）膜上对10皮摩尔的GM1进行串联质谱分析，我们能够确定详细的神经酰胺结构，即鞘氨醇碱基和脂肪酸结构，而用樱草灵染色时，该膜上仅可见微弱条带。检测限约为1皮摩尔的GM1，低于传统报告中的值（10皮摩尔）。
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