Downey Mark O, Rochfort Simone
Viticulture, Future Farming Systems Research, Department of Primary Industries Victoria, P.O. Box 905, Mildura, Vic. 3502, Australia.
J Chromatogr A. 2008 Aug 1;1201(1):43-7. doi: 10.1016/j.chroma.2008.06.002. Epub 2008 Jun 6.
A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyanins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis.
大规模葡萄栽培试验的一个局限在于，通过高效液相色谱法（HPLC）对果实进行全面成分分析所需的时间和成本。此外，通常需要采用不同的方法来鉴定和定量不同种类的代谢物。为了解决这些不足，开发了一种反相HPLC方法，用于同时分离葡萄皮中存在的花青素和黄酮醇。该方法采用用10%甲酸酸化的甲醇和水梯度，运行时间为48分钟，包括重新平衡。通过质谱分析确认了设拉子（Vitis vinifera L.）葡萄皮中花青素和黄酮醇的身份。
