Mulinacci N, Santamaria A R, Giaccherini C, Innocenti M, Valletta A, Ciolfi G, Pasqua G
Dipartimento di Scienze Farmaceutiche, Florence, Italy.
Nat Prod Res. 2008;22(12):1033-9. doi: 10.1080/14786410802133845.
The objective of the present study was to evaluate the amount of some potential health-promoting phenols in the grape of Vitis vinifera cv. Cesanese d'Affile and in wines made from these grapes. The analyses were performed using HPLC/DAD/MS. The accumulation of anthocyanins in the skin and flavan-3-ols in the seed was determined at different stages of ripening of the grape (i.e. green, veraison, middle stage of ripening, and complete ripening). Thirteen anthocyanins were identified in the skin at all stages of ripening, except the green stage. With regard to flavan-3-ols, (+)-catechin, (-)-epicatechin, and (-)-epicatechin gallate were detected in all of the seed samples. The highest (+)-catechin content was found in the seeds of the green grape (2 mg g(-1) DW), whereas in the seeds from the completely ripe grape the content was more than ten times lower. The highest catechin content in the seed was correlated with the lowest anthocyanin content in the skin. The wines produced in the years 2004 and 2005 showed, at wavelengths of 520 and 280 nm, almost identical quali-quantitative chromatographic profiles, with high concentrations of anthocyanin 3-O-glucosides, low concentrations of acylated anthocyanins, and trace amounts of (+)-catechin and (-)-epicatechin.
本研究的目的是评估欧亚种葡萄切萨内ese d'Affile的葡萄及用这些葡萄酿造的葡萄酒中一些潜在的促进健康的酚类物质的含量。分析采用高效液相色谱/二极管阵列检测器/质谱联用仪进行。在葡萄成熟的不同阶段（即绿色、转色期、成熟中期和完全成熟）测定了果皮中花色苷的积累和种子中黄烷-3-醇的积累。除绿色阶段外，在成熟的各个阶段的果皮中均鉴定出13种花色素苷。关于黄烷-3-醇，在所有种子样品中均检测到（+）-儿茶素、（-）-表儿茶素和（-）-表儿茶素没食子酸酯。绿色葡萄种子中的（+）-儿茶素含量最高（2毫克/克干重），而完全成熟葡萄种子中的含量则低十余倍。种子中儿茶素含量最高时，果皮中花色苷含量最低。2004年和2005年生产的葡萄酒在520和280纳米波长处显示出几乎相同的定性定量色谱图，花色苷3-O-葡萄糖苷浓度高，酰化花色苷浓度低，（+）-儿茶素和（-）-表儿茶素含量微量。
