Tao Li, Dong Hong-Jun, Chen Xi, Chen San-Feng, Wang Tian-Hong
National Key Laboratory for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing, 100094, People's Republic of China.
Appl Microbiol Biotechnol. 2008 Sep;80(4):573-8. doi: 10.1007/s00253-008-1562-7. Epub 2008 Jun 25.
The efe gene encoding an ethylene-forming enzyme from Pseudomonas syringae pv. glycinea has been expressed for the first time under the control of Trichoderma reesei cbh1 promoter in Trichoderma viride. Reverse transcription polymerase chain reaction analysis showed that transformant Y2 produced mRNA of the efe gene. Southern blot analysis showed that there was one copy of efe gene which was integrated into the chromosomal DNA of T. viride. Ethylene production by transformant Y2 was efficiently induced by cellulose, while very low level of ethylene was produced when sodium carboxymethyl cellulose or lactose was used as carbon source. Peptone exerted a much greater stimulatory effect on ethylene production. A high level of ethylene was produced when transformant Y2 was cultured in solid fermentation medium containing wheat straw, indicating that plant wastes could be directly converted to ethylene by the recombinant filamentous fungus.
编码来自大豆丁香假单胞菌pv. glycinea的乙烯形成酶的efe基因首次在里氏木霉cbh1启动子的控制下在绿色木霉中表达。逆转录聚合酶链反应分析表明，转化体Y2产生了efe基因的mRNA。Southern印迹分析表明，有一个efe基因拷贝整合到绿色木霉的染色体DNA中。转化体Y2的乙烯产生被纤维素有效诱导，而当使用羧甲基纤维素钠或乳糖作为碳源时，乙烯产生水平非常低。蛋白胨对乙烯产生具有更大的刺激作用。当转化体Y2在含有麦秸的固体发酵培养基中培养时产生了高水平的乙烯，表明植物废料可以被重组丝状真菌直接转化为乙烯。
