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在 中发现了五种新的乙烯形成酶，用于清洁生产乙烯。

Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene in .

机构信息

School of Environment and Energy, Peking University Shenzhen Graduate School, 2199 Lishui Rd., Shenzhen 518055, China.

出版信息

Int J Mol Sci. 2022 Apr 19;23(9):4500. doi: 10.3390/ijms23094500.

DOI:10.3390/ijms23094500
PMID:35562889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9101411/
Abstract

Ethylene is an essential platform chemical with a conjugated double bond, which can produce many secondary chemical products through copolymerisation. At present, ethylene production is mainly from petroleum fractionation and cracking, which are unsustainable in the long term, and harmful to our environment. Therefore, a hot research field is seeking a cleaner method for ethylene production. Based on the model ethylene-forming enzyme (Efe) AAD16440.1 (6vp4.1.A) from , we evaluated five putative Efe protein sequences using the data derived from phylogenetic analyses and the conservation of their catalytic structures. Then, pBAD expression frameworks were constructed, and relevant enzymes were expressed in BL21. Finally, enzymatic activity in vitro and in vivo was detected to demonstrate their catalytic activity. Our results show that the activity in vitro measured by the conversion of α-ketoglutarate was from 0.21-0.72 μmol ethylene/mg/min, which varied across the temperatures. In cells, the activity of the new Efes was 12.28-147.43 μmol/gDCW/h (DCW, dry cellular weight). Both results prove that all the five putative Efes could produce ethylene.

摘要

乙烯是一种具有共轭双键的重要平台化学品，可通过共聚反应生成许多次级化学产品。目前，乙烯的生产主要来自石油的分馏和裂解，从长远来看，这种方法是不可持续的，并且对我们的环境有害。因此，寻求一种更清洁的乙烯生产方法是一个热门的研究领域。基于 中的模型乙烯形成酶 (Efe) AAD16440.1 (6vp4.1.A)，我们使用来自系统发育分析和催化结构保守性的数据来评估了五个推定的 Efe 蛋白序列。然后，构建了 pBAD 表达框架，并在 BL21 中表达了相关酶。最后，通过体外和体内检测酶活性来证明它们的催化活性。我们的结果表明，通过α-酮戊二酸转化测量的体外活性为 0.21-0.72 μmol 乙烯/mg/min，其在不同温度下有所变化。在细胞中，新的 Efes 的活性为 12.28-147.43 μmol/gDCW/h（DCW，干细胞重量）。这两个结果都证明了所有五个推定的 Efes 都可以产生乙烯。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/639e78660b79/ijms-23-04500-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/c8c78c4e533d/ijms-23-04500-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/b00c919bf965/ijms-23-04500-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/918d5694ec33/ijms-23-04500-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/a3a4a3b66600/ijms-23-04500-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/03a21733921d/ijms-23-04500-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/62743b5794ca/ijms-23-04500-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/99d6b8b09c77/ijms-23-04500-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/58a08e21e853/ijms-23-04500-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/639e78660b79/ijms-23-04500-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/c8c78c4e533d/ijms-23-04500-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/b00c919bf965/ijms-23-04500-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/918d5694ec33/ijms-23-04500-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/a3a4a3b66600/ijms-23-04500-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/03a21733921d/ijms-23-04500-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/62743b5794ca/ijms-23-04500-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/99d6b8b09c77/ijms-23-04500-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/58a08e21e853/ijms-23-04500-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f49/9101411/639e78660b79/ijms-23-04500-g009.jpg

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