Matysik Silke, Herbarth Olf, Mueller Andrea
Department of Human Exposure Research and Epidemiology, Helmholtz Centre of Environmental Research, UFZ, Permoserstrasse 15, 04318, Leipzig, Germany.
J Microbiol Methods. 2008 Oct;75(2):182-7. doi: 10.1016/j.mimet.2008.05.027. Epub 2008 Jun 5.
Microbial volatile organic compounds (MVOCs) emitted from the mould species Penicillium expansum, P. chrysogenum, Aspergillus versicolor, A. fumigatus, A. niger and Cladosporium cladosporoides were analyzed by means of solid phase microextraction (SPME) and GCMS. The mould species were cultivated on the synthetic agar dichloran chloramphenicol (DG 18) and on wet wall paper. The production of MVOCs was monitored over several weeks to detect changes in the emission rates between the initial stage and later periods of growth. The cultivation on the synthetic agar resulted in MVOC patterns with a wide variety of signals. In contrast, the growth on wet wall paper led to changed MVOC patterns with less signals. The emission rates were drastically reduced. Components emitted by all six fungi species on wall paper were 2-pentanol and 2-pentanone. 1-Octen-3-ol was emitted by five fungi species. 2-Pentanol was only detected in considerable amounts during the first days of growth whereas 1-octen-3-ol had a more constant emission rate over the whole period of growth. On the basis of our studies some MVOCs could be proposed as specific for single fungi on wall paper, e.g. 1,3-dimethoxybenzene for A. versicolor and 2,4-pentandione for A. fumigatus.
采用固相微萃取（SPME）和气相色谱 - 质谱联用（GCMS）技术，对扩展青霉、产黄青霉、杂色曲霉、烟曲霉、黑曲霉和枝孢霉等霉菌产生的微生物挥发性有机化合物（MVOCs）进行了分析。这些霉菌在合成琼脂二氯苯胺氯霉素（DG 18）培养基和湿壁纸上进行培养。对MVOCs的产生情况进行了数周监测，以检测生长初期和后期排放速率的变化。在合成琼脂上培养产生的MVOCs图谱具有多种信号。相比之下，在湿壁纸上生长导致MVOCs图谱发生变化，信号减少，排放速率大幅降低。六种真菌在壁纸上排放的成分有2 - 戊醇和2 - 戊酮。五种真菌排放1 - 辛烯 - 3 - 醇。2 - 戊醇仅在生长初期的几天内大量检测到，而1 - 辛烯 - 3 - 醇在整个生长期间的排放速率更为稳定。基于我们的研究，一些MVOCs可被认为是壁纸上单一真菌的特异性排放物，例如杂色曲霉的1,3 - 二甲氧基苯和烟曲霉的2,4 - 戊二酮。
