Department of Sustainable Organic Chemistry and Technology, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium.
Fungal Biol. 2012 Sep;116(9):941-53. doi: 10.1016/j.funbio.2012.06.001. Epub 2012 Jun 30.
Microbial volatile organic compounds (MVOCs) were collected in water-damaged buildings to evaluate their use as possible indicators of indoor fungal growth. Fungal species isolated from contaminated buildings were screened for MVOC production on malt extract agar by means of headspace solid-phase microextraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. Some sesquiterpenes, specifically derived from fungal growth, were detected in the sampled environments and the corresponding fungal producers were identified. Statistical analysis of the detected MVOC profiles allowed the identification of species-specific MVOCs or MVOC patterns for Aspergillus versicolor group, Aspergillus ustus, and Eurotium amstelodami. In addition, Chaetomium spp. and Epicoccum spp. were clearly differentiated by their volatile production from a group of 76 fungal strains belonging to different genera. These results are useful in the chemotaxonomic discrimination of fungal species, in aid to the classical morphological and molecular identification techniques.
微生物挥发性有机化合物 (MVOCs) 被收集在受潮的建筑物中，以评估其作为室内真菌生长的可能指标的用途。从受污染建筑物中分离出的真菌种类通过顶空固相微萃取和气相色谱-质谱联用 (GC-MS) 分析，在麦芽提取物琼脂上筛选 MVOC 产生。在采样环境中检测到了一些倍半萜，特别是来源于真菌生长，并且鉴定了相应的真菌产生者。对检测到的 MVOC 谱的统计分析允许鉴定出特定于物种的 MVOC 或黑曲霉组、黄曲霉 ustus 和阿姆斯特丹青霉的 MVOC 模式。此外，Chaetomium spp. 和 Epicoccum spp. 通过它们与属于不同属的 76 株真菌菌株的挥发性产物的差异而明显区分。这些结果有助于真菌种的化学分类学鉴别，辅助经典的形态学和分子鉴定技术。
