Donalson L M, Kim W K, Chalova V I, Herrera P, McReynolds J L, Gotcheva V G, Vidanovic D, Woodward C L, Kubena L F, Nisbet D J, Ricke S C
Department of Poultry Science, Texas A&M University, College Station, TX 77843-2472, USA.
Poult Sci. 2008 Jul;87(7):1263-75. doi: 10.3382/ps.2007-00179.
The objective of this in vitro study was to evaluate the effects of combining a prebiotic with alfalfa on fermentation by laying hen cecal bacteria. Cecal contents from laying hens were diluted to a 1:3,000 concentration with an anaerobic dilution solution and added to serum tubes filled with ground alfalfa or a layer ration with or without fructooligosaccharide (FOS) prebiotic. Samples were processed in an anaerobic hood, pressurized by using a pressure manifold, and incubated at 37 degrees C. Volatile fatty acid (VFA) and lactic acid concentrations were quantified at 6 and 24 h of substrate fermentation. In this study, fermentation of alfalfa resulted in greater production of acetate, VFA, and lactic acid compared with the layer ration. Although with a relative inconsistency in data between trials, the amendment of FOS to both alfalfa and the layer ration appeared to further increase fermentation as demonstrated by overall higher propionate, butyrate, VFA, and lactic acid concentrations. The effect was more pronounced after 24 h of fermentation, implying time constraints for the optimal production of fermentation products in the chicken gastrointestinal tract. These data indicate that in vitro cecal fermentation can be enhanced by the addition of FOS.
这项体外研究的目的是评估益生元与苜蓿相结合对蛋鸡盲肠细菌发酵的影响。将蛋鸡的盲肠内容物用厌氧稀释液稀释至1:3000的浓度，并添加到装有磨碎苜蓿或含有或不含低聚果糖（FOS）益生元的蛋鸡日粮的血清管中。样品在厌氧箱中处理，使用压力歧管加压，并在37摄氏度下孵育。在底物发酵6小时和24小时时对挥发性脂肪酸（VFA）和乳酸浓度进行定量。在本研究中，与蛋鸡日粮相比，苜蓿发酵产生的乙酸、VFA和乳酸更多。尽管不同试验之间的数据存在相对不一致，但苜蓿和蛋鸡日粮中添加FOS似乎进一步增加了发酵，这表现为丙酸盐、丁酸盐、VFA和乳酸的总体浓度更高。发酵24小时后效果更明显，这意味着鸡胃肠道中发酵产物的最佳产生存在时间限制。这些数据表明，添加FOS可增强体外盲肠发酵。
