Caffier H, Brandau H
Arch Gynakol. 1978 Jul 19;225(3):255-65. doi: 10.1007/BF00667208.
Oestrogen and progesterone receptors were determined in 48 primary tumors of invasive mammary carcinoma. 86% showed a positive oestrogen receptor binding, 53% were progesterone receptor positive. The binding capacity for oestradiol was 5-211 pmoles/g tissue protein, for progestin (R 5020) 7-146 pmoles. The question of the investigation was whether the content of determined receptors depends on endogenous oestradiol and progesterone concentrations. A clear dependence did not exist between the hormone level in the plasma, collected just before the excision of the tumor, and the amount of identifiable receptors in the tumor. On the contrary, in considering the quantities of endogenous hormones in the tissues, based on the plasma content of the tissue and the plasma hormone level, a correlation could be found. The content of identifiable receptors decreased with increasing amounts of endogenous hormones in the cytosol. It is postulated that rather the amount of endogenous hormones in the tissue than the plasma hormone level characterizes the degree of saturation of existing receptors by endogenous hormones. Apparently, the determined receptor concentrations at endogenous hormone values under 2 pmoles/g tissue protein reflect rather the true receptor content, whereas at higher values too low or false negative results should be reckoned with.
对48例浸润性乳腺癌原发肿瘤进行了雌激素和孕激素受体检测。86%显示雌激素受体结合阳性，53%孕激素受体阳性。雌二醇的结合能力为5 - 211皮摩尔/克组织蛋白，孕激素（R 5020）为7 - 146皮摩尔。本研究的问题是所测定的受体含量是否取决于内源性雌二醇和孕激素浓度。在肿瘤切除前采集的血浆激素水平与肿瘤中可识别受体的数量之间不存在明显的相关性。相反，基于组织的血浆含量和血浆激素水平来考虑组织中内源性激素的量时，可以发现一种相关性。可识别受体的含量随着胞质溶胶中内源性激素量的增加而降低。据推测，表征现有受体被内源性激素饱和程度的是组织中内源性激素的量而非血浆激素水平。显然，当组织蛋白中内源性激素值低于2皮摩尔/克时所测定的受体浓度反映的是真正的受体含量，而当内源性激素值较高时，则应考虑到结果可能过低或出现假阴性。
