Panda Priyadarshi, Ali Shamsher, Lo Edward, Chung Bong Geun, Hatton T Alan, Khademhosseini Ali, Doyle Patrick S
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Lab Chip. 2008 Jul;8(7):1056-61. doi: 10.1039/b804234a. Epub 2008 May 22.
Encapsulating cells within hydrogels is important for generating three-dimensional (3D) tissue constructs for drug delivery and tissue engineering. This paper describes, for the first time, the fabrication of large numbers of cell-laden microgel particles using a continuous microfluidic process called stop-flow lithography (SFL). Prepolymer solution containing cells was flowed through a microfluidic device and arrays of individual particles were repeatedly defined using pulses of UV light through a transparency mask. Unlike photolithography, SFL can be used to synthesize microgel particles continuously while maintaining control over particle size, shape and anisotropy. Therefore, SFL may become a useful tool for generating cell-laden microgels for various biomedical applications.
将细胞包裹在水凝胶中对于构建用于药物递送和组织工程的三维（3D）组织构建体很重要。本文首次描述了使用一种称为停流光刻（SFL）的连续微流控工艺制造大量载细胞微凝胶颗粒的过程。含有细胞的预聚物溶液流过微流控装置，并通过透明掩膜使用紫外光脉冲反复定义单个颗粒阵列。与光刻不同，SFL可用于连续合成微凝胶颗粒，同时保持对颗粒大小、形状和各向异性的控制。因此，SFL可能成为一种用于为各种生物医学应用生成载细胞微凝胶的有用工具。
