Sabouri-Ghomi Mohsen, Wu Yi, Hahn Klaus, Danuser Gaudenz
Department of Cell Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Curr Opin Cell Biol. 2008 Oct;20(5):541-50. doi: 10.1016/j.ceb.2008.05.004. Epub 2008 Jun 27.
Understanding the structural adaptation and signaling of adhesion sites in response to mechanical stimuli requires in situ characterization of the dynamic activation of a large number of adhesion components. Here, we review high-resolution live cell imaging approaches to measure forces, assembly, and interaction of adhesion components, and the activation of adhesion-mediated signals. We conclude by outlining computational multiplexing as a framework for the integration of these data into comprehensive models of adhesion signaling pathways.
要理解粘附位点在响应机械刺激时的结构适应性和信号传导,需要对大量粘附成分的动态激活进行原位表征。在此,我们综述了用于测量粘附成分的力、组装和相互作用以及粘附介导信号激活的高分辨率活细胞成像方法。我们通过概述计算复用作为将这些数据整合到粘附信号通路综合模型中的框架来得出结论。