Department of Agricultural Chemistry, National Taiwan University, Taipei, Taiwan, Republic of China.
Biotechnol Bioeng. 1988 Sep 20;32(7):886-90. doi: 10.1002/bit.260320706.
Starchy agricultural wastes were inoculated with amylolytic yeasts for protein enrichment by solid-state fermentation. The moisture content of substrate was 65-69%, and water activity was equivalent to 0.98-0.99. The optimum conditions for protein enrichment were initial moisture content 65%, initial pH 4.5, a 1:1 mixture of ammonium sulfate and urea was incrementally added to the ferment with 1% added at zero time, 1% added at 24 h, and 0.5% added at 48 h, and incubation with amylolytic yeasts (1.0 x 10(10)/100 g substrate) at 30 degrees C for 2-3 days. The final product contained 16.11-20.82% protein.
淀粉质农业废弃物经固态发酵接种产朊假丝酵母进行蛋白质富集。发酵底物的水分含量为 65-69%，水活度相当于 0.98-0.99。蛋白质富集的最佳条件为初始水分含量 65%、初始 pH 值 4.5、以 1:1 的比例将硫酸铵和尿素混合物逐步添加到发酵物中，在 0 小时添加 1%、24 小时添加 1%、48 小时添加 0.5%，在 30°C 下用产朊假丝酵母（1.0 x 10(10)/100 g 底物）孵育 2-3 天。最终产物中含有 16.11-20.82%的蛋白质。
