Gomez-Gil Bruno, Fajer-Avila Emma, Pascual Javier, Macián M Carmen, Pujalte María J, Garay Esperanza, Roque Ana
CIAD, A.C., Mazatlán Unit for Aquaculture, AP. 711 Mazatlán, Sinaloa 82000, Mexico.
Int J Syst Evol Microbiol. 2008 Jul;58(Pt 7):1621-4. doi: 10.1099/ijs.0.65719-0.
Nine bacterial strains were studied by means of rep-PCR, 16S rRNA gene sequence analysis, DNA-DNA hybridization and physiological characterization. Typing analysis by means of rep-PCR showed that all nine strains were highly homogeneous, with similarities above 94 %. The strains were isolated from the same geographical area (Mazatlán, Sinaloa state, Mexico) and the same type of host (cultured rose snapper, Lutjanus guttatus), although from different individuals and organs. Comparison of the almost-complete 16S rRNA gene sequences of five strains showed that they belonged to the genus Vibrio and are closely related to the type strains of Vibrio brasiliensis and Vibrio hepatarius, with similarity values ranging from 97.9 to 98.1 % and from 97.4 to 97.8 %, respectively. The DNA-DNA hybridization value of strain CAIM 797(T) with the type strain of V. brasiliensis (CAIM 495(T)) was 47.5 %, with a reciprocal value of 44.7 %. The main phenotypic features of the strains were in agreement with the phylogenetic and genomic data. The results presented here support the description of a novel species, for which the name Vibrio sinaloensis sp. nov. is proposed, with strain CAIM 797(T) (=CECT 7298(T)) as the type strain.
通过重复聚合酶链反应（rep-PCR）、16S rRNA基因序列分析、DNA-DNA杂交和生理特征分析等方法对9株细菌进行了研究。rep-PCR分型分析表明，所有9株菌高度同源，相似性高于94%。这些菌株分离自同一地理区域（墨西哥锡那罗亚州马萨特兰）和同一类型的宿主（养殖的玫瑰笛鲷， Lutjanus guttatus），尽管来自不同个体和器官。对5株菌几乎完整的16S rRNA基因序列进行比较，结果表明它们属于弧菌属，与巴西弧菌（Vibrio brasiliensis）和肝弧菌（Vibrio hepatarius）的模式菌株密切相关，相似性值分别为97.9%至98.1%和97.4%至97.8%。菌株CAIM 797(T)与巴西弧菌模式菌株（CAIM 495(T)）的DNA-DNA杂交值为47.5%，反向值为44.7%。这些菌株的主要表型特征与系统发育和基因组数据一致。本文给出的结果支持一个新物种的描述，为此提出名为墨西哥弧菌（Vibrio sinaloensis sp. nov.）的新物种，菌株CAIM 797(T)（=CECT 7298(T)）为模式菌株。
