CIAD, A.C. Mazatlán Unit for Aquaculture and Environmental Management, AP. 711 Mazatlán, CP 82000 Sinaloa, México.
Institut de Reçerca i Tecnologia Agroalimentaries, Centre d'Aquicultura, Sant Carles de la Rapita, Spain.
Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2955-2961. doi: 10.1099/ijs.0.033191-0. Epub 2012 Jan 27.
Five strains (CAIM 1831(T), CAIM 1832, CAIM 1833, CAIM 1834 and CAIM 1836) were isolated from cultured sole (Solea senegalensis) in two regions of Spain, two strains (CAIM 404 and CAIM 1294) from wild-caught spotted rose snapper (Lutjanus guttatus) in Mexico, and one strain (CAIM 1835) from corals in Brazil. The 16S rRNA gene sequences of the novel isolates showed similarity to Vibrio ponticus (98.2-98.3%, GenBank accession no. AJ630103) and to a lesser degree to Vibrio furnissii (97.2-97.3%, X76336) and to Vibrio fluvialis (96.9-97.1%, X74703). Multilocus sequence analysis clustered these strains closely together and clearly separated them from phylogenetically related species of the genus Vibrio. Genomic fingerprinting by rep-PCR clustered the novel strains according to their geographical origin. Phenotypic analyses showed a large variation among the new strains, but many tests enabled them to be differentiated from other species of the genus Vibrio. The mean ΔT(m) values between the strains analysed here and closely related type strains were above 6.79 °C. The values between the novel isolates were below 2.35 °C, well outside the limit suggested for the delineation of a bacterial species. The phenotypic and genotypic data presented here clearly place these new strains as a coherent group within the genus Vibrio, for which we propose the name Vibrio alfacsensis sp. nov. with CAIM 1831(T) ( = DSM 24595(T) = S277(T)) as the type strain.
五个菌株（CAIM 1831(T)、CAIM 1832、CAIM 1833、CAIM 1834 和 CAIM 1836）从西班牙两个地区的养殖舌鳎中分离出来，两个菌株（CAIM 404 和 CAIM 1294）从墨西哥野生斑点鹦鲷中分离出来，一个菌株（CAIM 1835）从巴西的珊瑚中分离出来。新分离株的 16S rRNA 基因序列与 Vibrio ponticus（98.2-98.3%，GenBank 登录号 AJ630103）相似，与 Vibrio furnissii（97.2-97.3%，X76336）的相似度较低，与 Vibrio fluvialis（96.9-97.1%，X74703）的相似度较低。多位点序列分析将这些菌株聚类在一起，并与弧菌属的亲缘关系密切的种明显分开。基于重复序列 PCR 的基因组指纹图谱根据地理起源对新菌株进行聚类。表型分析显示新菌株之间存在很大差异，但许多试验使它们能够与弧菌属的其他种区分开来。与分析的菌株与亲缘关系密切的模式菌株的平均ΔT(m)值高于 6.79°C。新分离株之间的数值低于 2.35°C，远远超出建议用于划分细菌种的界限。这里呈现的表型和基因型数据清楚地将这些新菌株置于弧菌属内的一个连贯群体中，我们建议将其命名为 Vibrio alfacsensis sp. nov.，CAIM 1831(T)（=DSM 24595(T)=S277(T)）作为模式菌株。
