Ratajczak Mariusz Z, Zuba-Surma Ewa K, Shin Dong-Myung, Ratajczak Janina, Kucia Magda
Stem Cell Institute at James Graham Brown Cancer Center, Hoenig Endowed Chair in Cancer Biology, University of Louisville, Louisville, KY 40202, USA.
Exp Gerontol. 2008 Nov;43(11):1009-17. doi: 10.1016/j.exger.2008.06.002. Epub 2008 Jun 14.
Recently, we purified rare CXC chemokine receptor 4 expressing (CXCR4(+)) small stem cells (SCs) from the murine bone marrow (BM) that express markers characteristic for embryonic (E)SCs, epiblast (EP)SCs, and primordial germ cells (PGCs). We named these primitive cells very small embryonic-like (VSEL) SCs (VSELs). Our data indicate that VSELs are also present in many other organs in mice and that they may differentiate into cells from all three germ layers. Similar SCs were also isolated from human cord blood (CB) and mobilized peripheral blood (mPB). We hypothesize that VSELs are deposited during gastrulation and organogenesis in developing organs/tissues of mammals as a population of pluripotent stem cells (PSCs) that give rise to tissue committed monopotent SCs and that their number decreases with age. Therefore VSELs could play a pivotal role in normal rejuvenation of adult tissues as well as involvement in regeneration of damaged organs. Thus, these cells are potential SCs candidates for regenerative medicine and we envision that the regenerative potential of these cells could be harnessed to decelerate the aging processes.
最近，我们从小鼠骨髓（BM）中纯化出了罕见的表达CXC趋化因子受体4（CXCR4(+)）的小干细胞（SCs），这些细胞表达胚胎干细胞（ESCs）、上胚层干细胞（EPCs）和原始生殖细胞（PGCs）的特征性标志物。我们将这些原始细胞命名为非常小的胚胎样（VSEL）SCs（VSELs）。我们的数据表明，VSELs也存在于小鼠的许多其他器官中，并且它们可能分化为来自所有三个胚层的细胞。类似的SCs也从人脐带血（CB）和动员的外周血（mPB）中分离得到。我们推测，VSELs作为一群多能干细胞（PSCs），在哺乳动物发育中的器官/组织的原肠胚形成和器官发生过程中沉积下来，这些多能干细胞可产生组织定向的单能SCs，并且其数量会随着年龄的增长而减少。因此，VSELs可能在成体组织的正常年轻化以及受损器官的再生过程中发挥关键作用。因此，这些细胞是再生医学中潜在的干细胞候选者，我们设想可以利用这些细胞的再生潜力来延缓衰老过程。
