Fukushima K, Masuda T, Ohtani H, Sasaki I, Funayama Y, Matsuno S, Nagura H
First Department of Surgery, Tohoku University School of Medicine, Sendai, Japan.
Gastroenterology. 1991 Sep;101(3):670-8. doi: 10.1016/0016-5085(91)90524-o.
Phenotypic characterization and distribution of gamma/delta T lymphocytes in the intestinal mucosa were investigated in ulcerative colitis and Crohn's disease by immunohistochemistry. The ratio of delta(+) cells to CD3(+) cells in the intraepithelial space of colon was decreased in Crohn's disease (13%) and strikingly decreased in ulcerative colitis (8%) compared with the control (36%). Delta(+) cells in the lamina propria were also decreased, particularly in the distal ileum of Crohn's disease (4%), compared with the control (15%). On the contrary, the cells gathered at the severe inflammatory sites with other inflammatory cells, including beta(+) cells, and were densely distributed in the T-cell zone around lymphoid follicles. Phenotypic characterization showed that delta(+) lamina proprial lymphocytes of colon were mainly CD4(-)CD8(-) in the control (80%) and Crohn's disease (59%). However, in ulcerative colitis, CD4(-)CD8(-) delta(+) lymphocytes were rarely found (3%). This reflects the difference of immunologic background between the two diseases. Immunoelectron microscopically, these cells in inflammatory bowel disease were rich with vesicular structures in cytoplasms, whereas those in the control group contained electron-opaque granules. The decrease and the morphological change may be closely related to the weakness of mucosal defense.
通过免疫组织化学方法，对溃疡性结肠炎和克罗恩病患者肠黏膜中γ/δ T淋巴细胞的表型特征及分布进行了研究。与对照组（36%）相比，克罗恩病患者结肠上皮内间隙中δ(+)细胞与CD3(+)细胞的比例降低（13%），溃疡性结肠炎患者显著降低（8%）。固有层中的δ(+)细胞也减少，与对照组（15%）相比，尤其在克罗恩病患者的回肠末端（4%）。相反，这些细胞与包括β(+)细胞在内的其他炎症细胞聚集在严重炎症部位，并密集分布在淋巴滤泡周围的T细胞区。表型特征显示，对照组（80%）和克罗恩病患者（59%）结肠固有层中的δ(+)淋巴细胞主要为CD4(-)CD8(-)。然而，在溃疡性结肠炎中，很少发现CD4(-)CD8(-)δ(+)淋巴细胞（3%）。这反映了两种疾病免疫背景的差异。免疫电子显微镜观察发现，炎症性肠病中的这些细胞胞质内富含囊泡结构，而对照组中的细胞含有电子致密颗粒。这些细胞数量的减少和形态变化可能与黏膜防御功能减弱密切相关。
