糖苷酶敏感性：辛德毕斯病毒中糖蛋白寡糖分布的一种探针。
Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in Sindbis virus.
作者信息
McCarthy M, Harrison S C
出版信息
J Virol. 1977 Jul;23(1):61-73. doi: 10.1128/JVI.23.1.61-73.1977.
Abstract
Intact Sindbis virus and Triton-solubilized viral glycoprotein were treated with alpha-mannosidase and with a preparation of mixed glycosidases from Diplococcus pneumoniae to probe the accesibility of carbohydrate units on the viral surface. The products of glycosidase attack on Triton-solubilized virus showed that mose carbohydrate units of the glycoproteins are good substrates for these enzymes. The relative resistance of most of the viral oligosaccharides in intact virus particles showed that much of the carbohydrate is not accessible to glycosidases, probably because it is not exposed at the viral surface. The only completely accessible carbohydrate units on Sindbis glycoproteins were the type A oligosaccharides of E2. This differential accessibility of Sindbis oligosaccharides is discussed in relation to the organization of the viral surface.
摘要
用α-甘露糖苷酶和肺炎双球菌混合糖苷酶制剂处理完整的辛德毕斯病毒和经曲拉通溶解的病毒糖蛋白，以探究病毒表面碳水化合物单元的可及性。糖苷酶对经曲拉通溶解的病毒的作用产物表明，糖蛋白的大多数碳水化合物单元是这些酶的良好底物。完整病毒颗粒中大多数病毒寡糖的相对抗性表明，许多碳水化合物无法被糖苷酶作用，可能是因为它未暴露在病毒表面。辛德毕斯糖蛋白上唯一完全可及的碳水化合物单元是E2的A型寡糖。本文结合病毒表面的组织结构对辛德毕斯寡糖的这种差异可及性进行了讨论。
相似文献
1
Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in Sindbis virus.糖苷酶敏感性：辛德毕斯病毒中糖蛋白寡糖分布的一种探针。
J Virol. 1977 Jul;23(1):61-73. doi: 10.1128/JVI.23.1.61-73.1977.
2
Glycoproteins of Sindbis virus: priliminary characterization of the oligosaccharides.辛德毕斯病毒的糖蛋白：寡糖的初步表征
J Virol. 1974 Sep;14(3):522-30. doi: 10.1128/JVI.14.3.522-530.1974.
3
Growth-dependent alterations in oligomannosyl glycopeptides expressed in Sindbis virus glycoproteins.辛德毕斯病毒糖蛋白中表达的低聚甘露糖基糖肽的生长依赖性改变。
Biochemistry. 1980 Nov 25;19(24):5619-24. doi: 10.1021/bi00565a025.
4
Glucose trimming and mannose trimming affect different phases of the maturation of Sindbis virus in infected BHK cells.葡萄糖修剪和甘露糖修剪影响感染的BHK细胞中辛德毕斯病毒成熟的不同阶段。
Virology. 1987 Nov;161(1):37-44. doi: 10.1016/0042-6822(87)90168-1.
5
Growth of enveloped RNA viruses in a line of chinese hamster ovary cells with deficient N-acetylglucosaminyltransferase activity.包膜RNA病毒在N-乙酰葡糖胺基转移酶活性缺陷的中国仓鼠卵巢细胞系中的生长情况。
J Virol. 1975 Jan;17(1):239-46. doi: 10.1128/JVI.17.1.239-246.1976.
6
Biosynthesis of the Sindbis virus carbohydrates.辛德毕斯病毒碳水化合物的生物合成。
J Virol. 1973 Dec;12(6):1366-74. doi: 10.1128/JVI.12.6.1366-1374.1973.
7
Carbohydrate structure of Sindbis virus glycoprotein E2 from virus grown in hamster and chicken cells.在仓鼠和鸡细胞中生长的辛德毕斯病毒糖蛋白E2的碳水化合物结构。
J Virol. 1979 Feb;29(2):546-54. doi: 10.1128/JVI.29.2.546-554.1979.
8
Glycosylation of intracellular Sindbis virus glycoproteins.细胞内辛德毕斯病毒糖蛋白的糖基化作用。
Biochemistry. 1982 Apr 27;21(9):2140-5. doi: 10.1021/bi00538a024.
9
On the role of oligosaccharide trimming in the maturation of Sindbis and influenza virus.寡糖修剪在辛德毕斯病毒和流感病毒成熟过程中的作用
Arch Virol. 1984;81(1-2):25-39. doi: 10.1007/BF01309294.
10
Demonstration of an endo-glycosidase acting on a glycoprotein.一种作用于糖蛋白的内切糖苷酶的证明。
J Biol Chem. 1971 Sep 10;246(17):5535-7.
引用本文的文献
1
The surface structure of Venezuelan equine encephalomyelitis virus.委内瑞拉马脑炎病毒的表面结构
Arch Virol. 1980;63(2):165-9. doi: 10.1007/BF01320775.
2
The role of glycosylation on haemagglutination and immunological reactivity of rubella virus.
Arch Virol. 1984;79(3-4):139-46. doi: 10.1007/BF01310807.
3
Semliki Forest virus: a probe for membrane traffic in the animal cell.Semliki森林病毒：动物细胞中膜转运的一种探针。
Adv Protein Chem. 1984;36:79-132. doi: 10.1016/s0065-3233(08)60296-x.
4
Virion polypeptide heterogeneity among virulent and avirulent strains of eastern equine encephalitis (EEE) virus.
Arch Virol. 1981;68(3-4):229-37. doi: 10.1007/BF01314576.
5
Spikes and fimbriae: alpha-helical proteins form surface projections on microorganisms.刺突和菌毛：α-螺旋蛋白在微生物表面形成突起。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5303-4. doi: 10.1073/pnas.78.9.5303.
6
Hazelhurst-vesicular-stomatitis-virus G and Sindbis-virus E1 glycoproteins undergo similar host-cell-dependent variation in oligosaccharide processing.黑泽赫斯特水泡性口炎病毒G糖蛋白和辛德毕斯病毒E1糖蛋白在寡糖加工过程中经历相似的宿主细胞依赖性变异。
Biochem J. 1985 Jul 1;229(1):47-55. doi: 10.1042/bj2290047.
7
Investigation of the role of glycans for the biological activity of Semliki Forest virus grown in Aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming.
Arch Virol. 1988;102(1-2):73-89. doi: 10.1007/BF01315564.
8
Removal of carbohydrate from influenza A virus and its hemagglutinin and the effect on biological activities.甲型流感病毒及其血凝素中碳水化合物的去除及其对生物学活性的影响。
J Virol. 1978 Jul;27(1):164-71. doi: 10.1128/JVI.27.1.164-171.1978.
9
Carbohydrate structure of Sindbis virus glycoprotein E2 from virus grown in hamster and chicken cells.在仓鼠和鸡细胞中生长的辛德毕斯病毒糖蛋白E2的碳水化合物结构。
J Virol. 1979 Feb;29(2):546-54. doi: 10.1128/JVI.29.2.546-554.1979.
本文引用的文献
1
PRECIPITATION AND RECOVERY OF SINDBIS VIRUS FROM SOLUTIONS OF LOW IONIC STRENGTH.从低离子强度溶液中沉淀和回收辛德毕斯病毒
Virology. 1963 Oct;21:273-4. doi: 10.1016/0042-6822(63)90270-8.
2
PURIFICATION AND PARTIAL CHEMICAL ANALYSIS OF SINDBIS VIRUS.辛德毕斯病毒的纯化与部分化学分析
Virology. 1963 Jul;20:433-45. doi: 10.1016/0042-6822(63)90092-8.
3
The properties of a carbohydrate-amino acid complex from ovalbumin.来自卵清蛋白的碳水化合物-氨基酸复合物的性质。
J Biochem. 1962 May;51:359-64.
4
Carbohydrates in protein. 7. The nature of the carbohydrate in ovomucoid.蛋白质中的碳水化合物。7. 卵类粘蛋白中碳水化合物的性质。
Biochem J. 1963 Jun;87(3):581-5. doi: 10.1042/bj0870581.
5
The thiobarbituric acid assay of sialic acids.唾液酸的硫代巴比妥酸测定法。
J Biol Chem. 1959 Aug;234(8):1971-5.
6
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.节肢动物传播病毒的血凝及血凝抑制技术。
Am J Trop Med Hyg. 1958 Sep;7(5):561-73. doi: 10.4269/ajtmh.1958.7.561.
7
The membrane proteins of Semliki Forest virus have a hydrophobic part attached to the viral membrane.塞姆利基森林病毒的膜蛋白有一个附着在病毒膜上的疏水部分。
FEBS Lett. 1972 Dec 1;28(2):179-182. doi: 10.1016/0014-5793(72)80706-3.
8
Replication of Semliki Forest virus: an electron microscopic study.Semliki森林病毒的复制：一项电子显微镜研究。
Virology. 1967 May;32(1):128-43. doi: 10.1016/0042-6822(67)90261-9.
9
Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins.辛德毕斯病毒对鸡和仓鼠细胞的感染：病毒特异性蛋白的合成
Virology. 1969 Mar;37(3):367-76. doi: 10.1016/0042-6822(69)90220-7.
10
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
Zotero 插件