Yoon S J, Konstantinov K B
Miles Biotechnology, 4th & Parker Streets, P.O. Box 1986, Berkeley, CA 94701.
Biotechnol Bioeng. 1994 Oct;44(8):983-90. doi: 10.1002/bit.260440815.
A new method for real-time monitoring of the oxygen uptake rate (OUR) in bioreactors, based on dissolved oxygen (DO) measurement at two points, has been developed and tested extensively. The method has several distinct advantages over known techniques.It enables the continuous and undisturbed monitoring of OUR, which is conventionally impossible without gas analyzers. The technique does not require knowledge of k(L)a. It provides smooth, robust, and reliable signal. The monitoring scheme is applicable to both microbial and mammalian cell bioprocesses of laboratory or industrial scale. The method was successfully used in the cultivation of NSO-derived murine myeloma cell line producing monoclonal antibody. It was found that while the OUR increased with the cell density, the specific OUR decreased to approximately one-half at cell concentrations of 16 x 10(6) cells/mL, indicating gradual reduction of cell respiration activity. Apart from the laboratory scale cultivation, the method was applied to industrial scale perfusion culture, as well as to processes using other cell lines. (c) 1994 John Wiley & Sons, Inc.
一种基于两点溶解氧（DO）测量的生物反应器中氧摄取率（OUR）实时监测新方法已被开发并广泛测试。该方法与已知技术相比有几个明显优势。它能够对OUR进行连续且不受干扰的监测，而传统上若无气体分析仪这是不可能实现的。该技术不需要了解k(L)a。它能提供平滑、稳健且可靠的信号。该监测方案适用于实验室规模或工业规模的微生物和哺乳动物细胞生物过程。该方法已成功用于培养产生单克隆抗体的NSO衍生小鼠骨髓瘤细胞系。研究发现，虽然OUR随细胞密度增加，但在细胞浓度为16×10(6) 个细胞/mL时，比OUR降至约一半，表明细胞呼吸活性逐渐降低。除实验室规模培养外，该方法还应用于工业规模灌注培养以及使用其他细胞系的过程。(c) 1994 John Wiley & Sons, Inc.
