Chu E H, Stjernswärd J, Clifford P, Klein G
Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, USA.
J Natl Cancer Inst. 1967 Oct;39(4):595-617.
Human peripheral lymphocytes from tumor-bearing and non-tumor-bearing patients were added to monolayer cultures of autochthonous and allogeneic normal or neoplastic cells in vitro with or without phytohemagglutinin (PHA). The normal cells were derived from skin, the tumor cells from postnasal carcinomas or sarcomas. The cultures were scored for clearly visible plaques in the monolayer. Without PHA, lymphocytes affected autochthonous skin target cells in 1 of 16 cultures. If PHA was added,the figure increased to nearly 50%(12/28). Whether this phenomenon is related to an autoimmune reaction or to some less specific effect of the PHA stimulus is unknown at present. In the absence of PHA, lymphocytes from African tumor-bearing hosts destroyed allogeneic skin fibroblasts in 4 of 14 cases, and lymphocytes from non-tumor-bearing Swedish donors showed this effect in 14 of 24 tests. The somewhat lower reactivity of the African lymphocytes was also apparent in other tests. It cannot be stated whether the difference was due to the tumor-bearing condition of the hosts or to some other differences involved in comparison of the two groups. Without PHA, lymphocytes of the African tumor patients destroyed their own autochthonous tumor cultures in 4 of 16 cases. Addition of PHA increased the proportion of positives to 20 of 28. A comparison with the corresponding figures for autochthonous skin cultures (1/16 and 12/28, respectively) indicates a relatively higher reactivity against the autochthonous tumor cells, both with and without PHA. This cannot be interpreted as a difference in target cell sensitivity to the same lymphocyte action, because no such difference was apparent when the sensitivity of skin and tumor to allogeneic lymphocytes was compared in the absence of PHA (4/14 and 4/14 with African lymphocyte donors; 14/24 and 14/24 with Swedish lymphocyte donors, respectively). The most probable explanation is that the antigenic barrier responsible for the lymphocyte effect is larger for tumor than for skin, or that the results reflect a presensitization of the tumor donor against its autochthonous neoplastic cells.
将荷瘤患者和非荷瘤患者的人外周淋巴细胞，添加到自体和异体正常或肿瘤细胞的单层培养物中，体外培养时添加或不添加植物血凝素（PHA）。正常细胞来源于皮肤，肿瘤细胞来源于鼻咽癌或肉瘤。对单层培养物中清晰可见的噬斑进行计数。不添加PHA时，16个培养物中有1个培养物中的淋巴细胞对自体皮肤靶细胞产生了影响。如果添加PHA，这一比例增加到近50%（12/28）。目前尚不清楚这种现象是与自身免疫反应有关，还是与PHA刺激的一些不太特异的效应有关。不添加PHA时，14例中有4例来自非洲荷瘤宿主的淋巴细胞破坏了异体皮肤成纤维细胞，24次试验中有14次来自非荷瘤瑞典供体的淋巴细胞表现出这种效应。非洲淋巴细胞的反应性略低在其他试验中也很明显。尚不能确定这种差异是由于宿主的荷瘤状态，还是由于两组比较中涉及的其他差异。不添加PHA时，16例中有4例非洲肿瘤患者的淋巴细胞破坏了他们自己的自体肿瘤培养物。添加PHA后，阳性比例增加到28例中的20例。与自体皮肤培养物的相应数据（分别为1/16和12/28）比较表明，无论有无PHA，对自体肿瘤细胞的反应性相对较高。这不能解释为靶细胞对相同淋巴细胞作用的敏感性差异，因为在不添加PHA的情况下比较皮肤和肿瘤对异体淋巴细胞的敏感性时，没有明显差异（非洲淋巴细胞供体时分别为4/14和4/14；瑞典淋巴细胞供体时分别为14/24和14/24）。最可能的解释是，负责淋巴细胞效应的抗原屏障对肿瘤比对皮肤更大，或者结果反映了肿瘤供体对其自体肿瘤细胞的预致敏。
