Baioni C S, de Souza C M, Ribeiro Braosi A P, Luczyszyn S M, Dias da Silva M A, Ignácio S A, Naval Machado M A, Benato Martins W D, Riella M C, Pecoits-Filho R, Trevilatto P C
Dentistry, Pontifical Catholic University of Parana (PUCPR), Rua Imaculada Conciicao, Curitibua, SP, Brazil.
J Periodontal Res. 2008 Oct;43(5):578-84. doi: 10.1111/j.1600-0765.2008.01098.x. Epub 2008 Jul 4.
Chronic kidney disease (CKD) is a complex disorder, which results in several complications involving disturbance of mineral metabolism. Periodontal disease is an infectious disease that appears to be an important cause of systemic inflammation in CKD patients. Periodontal disease is characterized by clinical attachment loss (CAL) caused by alveolar bone resorption around teeth, which may lead to tooth loss. Osteoprotegerin (OPG) is a key regulator of osteoclastogenesis. Polymorphisms are the main source of genetic variation, and single nucleotide polymorphisms (SNPs) have been reported as major modulators of disease susceptibility. The aim of this study was to investigate the association of a polymorphism located at position -223 in the untranslated region of the OPG gene, previously known as -950, with susceptibility to CKD and periodontal disease.
A sample of 224 subjects without and with CKD (in hemodialysis) was divided into groups with and without periodontal disease. The OPG polymorphism was analyzed by polymerase chain reaction and restriction fragment length polymorphism.
No association was found between the studied OPG polymorphism and susceptibility to CKD or periodontal disease.
It was concluded that polymorphism OPG-223 (C/T) was not associated with CKD and periodontal disease in a Brazilian population. Studies on other polymorphisms in this and other genes of the host response could help to clarify the involvement of bone metabolism mediators in the susceptibility to CKD and periodontal disease.
慢性肾脏病（CKD）是一种复杂的病症，会引发多种并发症，包括矿物质代谢紊乱。牙周病是一种传染病，似乎是CKD患者全身炎症的重要原因。牙周病的特征是牙齿周围牙槽骨吸收导致临床附着丧失（CAL），这可能导致牙齿脱落。骨保护素（OPG）是破骨细胞生成的关键调节因子。多态性是遗传变异的主要来源，单核苷酸多态性（SNP）已被报道为疾病易感性的主要调节因子。本研究的目的是调查位于OPG基因非翻译区 -223 位置（以前称为 -950）的多态性与CKD和牙周病易感性之间的关联。
选取224名未患CKD和正在进行血液透析的CKD患者作为样本，分为有牙周病组和无牙周病组。通过聚合酶链反应和限制性片段长度多态性分析OPG多态性。
在所研究的OPG多态性与CKD或牙周病易感性之间未发现关联。
得出的结论是，在巴西人群中，OPG -223（C/T）多态性与CKD和牙周病无关。对宿主反应中该基因和其他基因的其他多态性进行研究，可能有助于阐明骨代谢介质在CKD和牙周病易感性中的作用。
