Choi Wonshik, Yu Chung-Chieh, Fang-Yen Christopher, Badizadegan Kamran, Dasari Ramachandra R, Feld Michael S
G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge,Massachusetts 02139, USA.
Opt Lett. 2008 Jul 15;33(14):1596-8. doi: 10.1364/ol.33.001596.
We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.
我们对单个活细胞进行基于场的角分辨光散射测量。我们使用激光干涉仪在图像平面获取细胞的相位和振幅图像。角散射光谱由透过细胞的场的傅里叶变换计算得出。使用层析相显微镜测量同一细胞的并发三维折射率分布。通过测量单个细胞在接触乙酸期间光散射的瞬态增加，我们将单个细胞的散射特性与其折射率分布相关联，并表明结果与基于玻恩近似的模型高度吻合。
