健康红细胞和疟原虫感染红细胞的静态和动态光散射。
Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells.
机构信息
Massachusetts Institute of Technology, G. R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
出版信息
J Biomed Opt. 2010 Mar-Apr;15(2):020506. doi: 10.1117/1.3369966.
Abstract
We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.
摘要
我们展示了个体疟原虫感染的人类红细胞（Pf-RBC）的光散射，并证明了由诱导疟疾的寄生虫发育引起的散射信号的逐渐变化。通过使用定量相位显微镜和傅里叶变换光散射技术选择性地对电场进行成像，我们计算了单个 Pf-RBC 的光散射图谱。我们表明，通过静态散射图谱可以清楚地识别 Pf-RBC 的病理状态的发生和进展。从动态光散射中可以捕捉到 Pf-RBC 膜的生物物理性质的渐进变化。
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