Brannetti B, Zanzoni A, Montecchi-Palazzi L, Cesareni G, Helmer-Citterich M
Department of Biology, University of Rome Tor Vergata, Rome, Italy.
Comp Funct Genomics. 2001;2(5):314-8. doi: 10.1002/cfg.104.
Methods that aim at predicting interaction partners are very likely to play an important role in the interpretation of genomic information. iSPOT (iSpecificity Prediction Of Target) is a web tool (accessible at http://cbm.bio.uniroma2.it/iSPOT) developed for the prediction of protein-protein interaction mediated by families of peptide recognition modules. iSPOT accesses a database of position specific residue-residue interaction frequencies for members of the SH3 and PDZ protein domain families. The software utilises this database to provide a score for any potential domain peptide interaction.ISPOT: 1. evaluates the likelihood of the interaction between any of the peptides contained in an input protein and a list of domains of the two different families; 2. searches in the SWISS-PROT database for potential partners of a query domain; and 3. has access to a repository of all the domain/target peptide interaction data.
旨在预测相互作用伙伴的方法很可能在基因组信息的解读中发挥重要作用。iSPOT（靶标的特异性预测）是一个网络工具（可通过http://cbm.bio.uniroma2.it/iSPOT访问），用于预测由肽识别模块家族介导的蛋白质-蛋白质相互作用。iSPOT访问SH3和PDZ蛋白质结构域家族成员的位置特异性残基-残基相互作用频率数据库。该软件利用此数据库为任何潜在的结构域-肽相互作用提供一个分数。iSPOT：1. 评估输入蛋白质中包含的任何肽与两个不同家族的结构域列表之间相互作用的可能性；2. 在SWISS-PROT数据库中搜索查询结构域的潜在伙伴；3. 可访问所有结构域/靶标肽相互作用数据的存储库。
