奇异变形杆菌临床分离株中KPC-2的检测及首例关于奇异变形杆菌中KPCβ-内酰胺酶引起碳青霉烯类耐药的报道。
Detection of KPC-2 in a clinical isolate of Proteus mirabilis and first reported description of carbapenemase resistance caused by a KPC beta-lactamase in P. mirabilis.
作者信息
Tibbetts R, Frye J G, Marschall J, Warren D, Dunne W
机构信息
Department of Pathology and Immunology, Division of Laboratory Medicine, Washington University School of Medicine, St Louis, Missouri 63122, USA.
出版信息
J Clin Microbiol. 2008 Sep;46(9):3080-3. doi: 10.1128/JCM.00979-08. Epub 2008 Jul 16.
Abstract
An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase gene produced an amplicon of the expected size which was confirmed to be bla(KPC-2) by sequence analysis. Transformation of a susceptible Escherichia coli host with plasmid preparations from the isolate generated a transformant for which the MICs of all of the carbapenems tested were increased three- to fourfold. We believe this to be the first report of carbapenem resistance in P. mirabilis caused by the acquisition of bla(KPC).
摘要
从一名糖尿病患者血培养物中分离出的奇异变形杆菌，经纸片扩散药敏试验显示对亚胺培南、美罗培南和厄他培南耐药。用针对bla(KPC)碳青霉烯酶基因的特异性引物对从该分离株中回收的全细胞和/或质粒DNA进行扩增，产生了预期大小的扩增子，经序列分析证实为bla(KPC-2)。用该分离株的质粒提取物转化敏感的大肠杆菌宿主，产生了一个转化体，其对所有测试碳青霉烯类药物的最低抑菌浓度（MIC）增加了三到四倍。我们认为这是奇异变形杆菌因获得bla(KPC)而导致碳青霉烯耐药的首次报道。
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