Dubé Etienne, Shareck François, Hurtubise Yves, Beauregard Marc, Daneault Claude
Département de chimie-biologie, Université du Québec à Trois-Rivières, 3351 Des Forges, C.P. 500, Trois-Rivières, Quebec, Canada.
J Ind Microbiol Biotechnol. 2008 Oct;35(10):1123-9. doi: 10.1007/s10295-008-0391-0. Epub 2008 Jul 17.
Colored wastewater from textile industries is a consequence of dye manufacturing processes. Two percent of dyes that are produced are discharged directly in aqueous effluent and more than 10% are subsequently lost during the textile coloration process. It is not surprising that these compounds have become a major environmental concern. In that context, we have evaluated the potential use of Streptomyces coelicolor laccase for decolourization of various dyes with and without a mediator. Results showed that in all cases the combination of laccase and the mediator acetosyringone was able to rapidly decolourize, to various degrees, all the dyes tested. In 10 min, decolourization was achieved at 94% for acid blue 74, 91% for direct sky blue 6b and 65% for reactive black 5. Furthermore, decolourization was achieved at 21% for reactive blue 19 and at 39% for the direct dye Congo red in 60 min. These results demonstrate the potential use of this laccase in combination with acetosyringone, a natural mediator, for dye decolourization.
纺织工业产生的有色废水是染料制造过程的结果。所生产的染料中有2%直接排放到废水当中，超过10%在纺织染色过程中随后流失。这些化合物成为主要的环境问题也就不足为奇了。在此背景下，我们评估了天蓝色链霉菌漆酶在有或没有介体的情况下对各种染料进行脱色的潜在用途。结果表明，在所有情况下，漆酶与介体乙酰丁香酮的组合能够在不同程度上迅速使所有测试染料脱色。在10分钟内，酸性蓝74的脱色率达到94%，直接湖蓝6b的脱色率达到91%，活性黑5的脱色率达到65%。此外，活性蓝19在60分钟内的脱色率为21%，直接染料刚果红在60分钟内的脱色率为39%。这些结果证明了这种漆酶与天然介体乙酰丁香酮结合用于染料脱色的潜在用途。
